摘要
本研究对金针菇淀粉酶家族基因进行了信息分析,并选用金针菇双核菌株H1123作为实验材料,分析了菌丝生长过程中淀粉酶活性和淀粉酶基因表达特性之间的关系。结果表明,金针菇淀粉酶家族包含6个α淀粉酶和1个γ淀粉酶。7个淀粉酶基因的表达量均在菌丝接种后第10天出现峰值,并与胞外淀粉酶活性呈同步变化,说明基质中淀粉的分解和利用是淀粉酶家族各成员之间相互协调的结果。其中α-Amy-1、α-Amy-4、α-Amy-5的上调幅度最大,为淀粉降解和代谢过程的主效基因。值得注意的是胞内淀粉酶基因α-Amy-1在第10天时达到约90倍的上调表达水平。我们推测:金针菇胞外淀粉酶将淀粉分解为小分子单糖的同时,其胞内淀粉酶也参与了这些糖类的吸收和运输过程。
The amylase family genes in the genome of Flammulina velutipes were identified and analyzed. The correlation between the expression levels of amylase family genes and the activity of amylase were analyzed in the F. velutipes dikaryotic strain H1123. It was turned out that there were six α-amylases and one γ-amylase in F. velutipes. All the amylase genes showed the highest expression levels on the 10 th day after mycelium incubation, being consistently correlated with the activity of extracellular amylase. The results indicated that the degradation and utilization of starch in medium was the result of mutual coordination among amylase family members. Amylase genes α-Amy-1, α-Amy-4, α-Amy-5 were the main genes functioning in starch degradation and metabolism due to their highest up-regulated expression levels. It was worthy of note that the up-regulated expression level of the intracellular enzyme gene α-Amy-1 multiplied about 90 times in 10 days after inoculation,suggesting that the intracellular amylases were also involved in the absorption and transportation of monosaccharides during the process that extracellular amylases decomposed starch into monosaccharide in F. velutipes.
出处
《菌物学报》
CAS
CSCD
北大核心
2016年第7期865-873,共9页
Mycosystema
基金
国家重点基础研究发展计划(2014CB138302)
福建省现代农业食用菌产业技术体系项目(K83139295)~~
关键词
金针菇
淀粉酶
酶活
基因表达
相关分析
Flammulina velutipes
amylase
enzyme activity
gene expression
correlation analysis
