摘要
目的：研究MTB潜伏感染者特异蛋白标志物的血浆蛋白质组学，寻找MTB潜伏感染的特异性蛋白标志物。方法应用非标记（ label－free）定量蛋白质组学技术检测15例MTB潜伏感染者和15名健康对照者血浆蛋白质谱，应用基因本体论（GO）、京都基因与基因组百科全书（KEGG）和BiNGO等分析软件对差异蛋白质进行生物信息学分析。组间差异分析采用t检验。结果 MTB潜伏感染组与健康组共发现23个差异表达蛋白质，MTB潜伏感染者中15个表达下调（差异倍数＜0.5， P＜0.05），8个表达上调（差异倍数＞2，P＜0.05）。生物信息学分析发现α1蛋白酶抑制剂（alpha－1 protease inhibitor，AAT）、C3和C4A在MTB潜伏感染组与健康组表达具有差异性。结论 MTB潜伏感染者与健康人血浆蛋白质谱存在差异，蛋白AAT、C3和C4A有望成为区分MTB潜伏感染和健康人的蛋白标志物。
Objective To screen specific biomarkers for latent tuberculosis infection by comparing the plasma proteomic profiling between latent tuberculosis infection and healthy controls .Methods The plasma proteins from 15 cases with latent tuberculosis infection and 15 healthy controls were detected by the label-free quantitative proteomic technology .Differential expressed proteins were analyzed by GO , KEGG, and BiNGO analysis.Student’s t test was used to analyze the differential expression between 2 groups. Results Twenty-three candidate proteins were identified , among which 15 proteins were downregulated （〈0.5-fold at P 〈0.05 ） and 8 proteins were upregulated （ 〉2.0-fold at P 〈0.05 ） in the latent tuberculosis infection group.Bioinformatic analysis revealed 3 proteins AAT, C3 and C4A to be the most significant.Conclusion There were differential plasma protein profiles between latent tuberculosis infection and healthy controls.Candidate proteins AAT, C3 and C4A were promising biomarkers for discriminating cases with latent tuberculosis infection from healthy persons.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2016年第7期519-523,共5页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
“十二五”国家科技重大专项（2015ZX10004801-003）
北京市医院管理局“青苗”计划专项（QML20151501）
重大传染病防治协同创新中心（PXM2015_014226_000011）
北京市医院管理局临床医学发展专项（ZYLX201304）
