摘要
目的预测结核分枝杆菌潜伏感染相关蛋白Rv2029c的B/T细胞抗原表位,分析抗原位点的氨基酸变异。方法 BLAST在线分析Rv2029c与人类蛋白同源性,利用DNAstar软件包中的Protean软件预测Rv2029c潜在B/T细胞抗原表位,通过BioEdit软件比对NCBI数据库中所有结核分枝杆菌复合群Rv2029c氨基酸序列,找出变异位点。结果 Rv2029c与人类蛋白同源性较低。预测该蛋白共含有12个潜在B细胞抗原表位,分别位于5-13、32-49、85-88、97-102、106-113、147-161、165-172、178-183、220-227、262-264、318-326、334-339位氨基酸;含有6个T细胞表位数,分别位于23-36、53-66、135-170、207-223、271-289、307-318位氨基酸。除136和221位氨基酸外,不同地区来源的结核杆菌分离株Rv2029c蛋白氨基酸序列较少发生变异。结论结核分枝杆菌Rv2029c是一个B细胞抗原表位占优势的蛋白抗原,T细胞表位略少,且氨基酸序列较为保守,可作为结核监测、治疗、预防的新靶点。
Objective To predict the epitopes of Mycobacterium tuberculosis dormancy-related protein Rv2029 cand to analyze amino acid variations in Rv2029 c. Methods The homology of Rv2029 cand human proteins was analyzed using online BLAST alignment.B-cell and T-cell epitopes of the Rv2029 cprotein were predicted using Protean in DNAStar.Amino acid variations in Rv2029 cwas analyzed via sequence alignment of Mycobacterium tuberculosis complex Rv2029 cin the NCBI database with the software BioEdit. Results Rv2029 chad little similarity to human proteins.There were 12 potential B-cell epitopes at amino acids 5-13,32-49,85-88,97-102,106-113,147-161,165-172,178-183,220-227,262-264,318-326,and 334-339,while 6T-cell epitopes were predicted to exist at amino acids 23-36,53-66,135-170,207-223,271-289,307-318.There were few amino acid variations in Rv2029 cof Mycobacterium tuberculosis isolates from different regions except for amino acids 136 and 221. Conclusion Rv2029 c,which had many potential B-cell epitopes,few T-cell epitopes,and a conserved amino acid sequence,could be a potential target for diagnostic agents or vaccines against tuberculosis.
作者
李宁
孟祥英
孙誉芳
乔晋娟
LINing;MENG Xiang-ying;SUN Yu-fang;QIAO Jin-juan(Department of Medical Laboratory Science,Wei fang Medical University,Wei fang ,Shandong ,China 261053)
出处
《中国病原生物学杂志》
CSCD
北大核心
2018年第12期1347-1351,1356,共6页
Journal of Pathogen Biology
基金
山东省自然科学基金项目(No.ZR2017LH057
ZR2016BL19)
