摘要
目的检测可手术肺腺癌组织中主要组织相容性复合体－Ⅰ类分子链相关蛋白A和B（MICA/B蛋白）的表达，探讨其临床意义。方法回顾分析郑州人民医院2002年1月至于2003年12月诊治的可手术肺腺癌100例。免疫组织化学染色法检测其MICA/B蛋白的表达，按照免疫组织化学染色评分标准≥5分者为高表达组，〈5分者为低表达组。运用χ2检验分析MICA/B蛋白与患者临床病理特征的关系。Kaplan－Meier生存曲线及Log－rank检验计算MICA/B蛋白表达与可手术肺腺癌总生存期之间的关系。采用Cox回归模型分析各个协变量单一及联合效应。结果MICA/B蛋白在可手术肺腺癌组织中的高表达率为38%（38/100）。MICA/B蛋白表达在EGFR基因突变组中的高表达率显著高于EGFR基因野生组（93.8%比11.8%，P〈0.001），而在患者不同年龄、性别、TNM分期、T分期、组织学分级及淋巴结转移之间差异均无统计学意义。MICA/B蛋白高表达组的总生存期显著短于低表达组（10.4比28.9个月，P＝0.005）。结论 可手术肺腺癌组织中MICA/B蛋白高表达，与EGFR基因突变型及总生存期有密切关系，有可能是可手术肺腺癌患者预后不良的指标。
Objective To explore the expression of major histocompatibility complex class Ⅰ chainrelated protein A and B （MICA/B） in operable lung adenocarcinoma and its clinical significance. Methods Between January 2002 and December 2003, 100 patients with operable lung adenocarcinoma in People＇ s Hospital of Zhengzhou were collected. The expression of MICA/B was examined by immunohistochemistry staining. According to immunohistochemical staining, the cases with score ≥5 points were high expression of MICA/B while 〈 5 points were low expression of MICA/B. Chi-square test was utilized to analyze the relationship between MICA/B expression and clinieopathologic features. The association between MICA/B protein and overall survival in the patients with operable lung adenocareinoma was analyzed by Kaplan-Meier survival curve, together with Log-Rank test. The COX regression model was established to analyze the single and combined effects of these covariants. Results The percentage with high expression of MICA/B protein in operable lung adenocareinoma tissue was 38% （38/100）. The over-expression rate of MICA/B protein in the group with mutant epidermal growth factor receptor （EGFR） gene was significantly higher than that in the group with wild EGFR gene （93.8% vs 11.8%, P〈0. 001 ）. No statistical significance was observed between the expression of MICA/B protein and other elinieopathologic parameters, including age, sex, TNM stage, T- staging, histological grade and lymph node metastasis. Kaplan-Meier analysis showed that overexpression of MICA/B protein was closely associated with shorter survival time （ 10.4 vs 28.9 months, P = 0. 005 ）. Conclusion Overexpression of MICA/B in operable lung adenocarcinoma tissue is closely related to the mutations of EGFR and overall survival, which may be a poor prognosis indicator in patients with operable lung adenocarcinoma.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2016年第26期2081-2085,共5页
National Medical Journal of China
基金
郑州市科技计划项目（121PLJRC532）
