摘要
利用毛细管电泳-紫外吸收法测定乙醇酸二聚物及其水解产物乙醇酸的浓度随时间变化的规律,建立新的数学模型,将水解反应转化率与乙醇酸二聚物及乙醇酸峰面积直接关联,进而计算乙醇酸二聚物在50 mmol·L^(-1)磷酸盐缓冲溶液中,不同温度时的水解反应速率常数。在343,353,363 K时,乙醇酸二聚物的水解反应速率常数分别为4.8×10^(-3),9.6×10^(-3),1.85×10^(-2)min^(-1)。根据阿仑尼乌斯公式计算得该反应的表观活化能为69.8 kJ·mol^(-1)。
The concentration of oligo（glycolic acid）2 （OGA-2） and its hydrolysis product glycolic acid （GA） were monitored by capillary electrophoresis with UV detection. A new mathematical model was used to build the relationship between the reaction conversion and peak areas of OGA-2 and GA, and then calculate the reaction rate constants of diglyeollic acid hydrolysis in 50 mmol · L^-1 phosphate buffer at varied temperatures. At 343, 353, 363 K, the measured rate constants of diglycollic acid were 4. 8 × 10^-3, 9. 6 × 10^-3, 1. 85 × 10^-2 min^-1, respectively. The apprarent activation energy of the hydrolysis was calculated to be 69. 8 kJ · mol^-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2016年第7期756-760,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
毛细管电泳法
乙醇酸二聚物
反应速率常数
水解
Capillary electrophoresis
Oligo（glycolic acid）2
Reaction rate constant
Hydrolysis
