摘要
目的探讨RAD52、XRCC2、XRCC4基因多态性与1,3-丁二烯(BD)作业工人DNA修复能力的关系。方法利用染色体断裂试验评价60名BD职业暴露工人和60名非暴露工人的外周血淋巴细胞对诱变剂博莱霉素(BLM)所致DNA损伤的修复能力,聚合酶链反应-限制性片段长度多态性方法分析RAD52、XRCC2和XRCC4基因多态性。结果职业BD暴露组染色体断裂率[(1.06±0.41)%]显著高于对照组[(0.85±0.36)%](P<0.01);暴露组中XRCC4 A245G位点AA基因型个体b/c率[(1.18±0.48)%]显著高于GG或AG+GG基因型个体[(0.91±0.29)%](P=0.02);XRCC4 T1394G位点TT基因型个体b/c率[(0.75±0.36)%]显著低于GG基因型个体[(1.13±0.42)%](P=0.04)。结论 XRCC4基因多态性与BD作业工人DNA修复能力存在相关性。
Objective To explore the relation between genetic polymorphisms of RAD52, XRCC2, XRCC4 and DNA repair capacity （DRC） in 1,3-butadiene （BD） workers. Methods DRC of DNA damage caused by bleomycin in peripheral blood lymphoeytes of 60 BD-exposed workers and 60 non-BD exposed workers were measured respectively, using chromatid breaks test, and the polymorphism of RAD52, XRCC2, XRCC4 were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. Results The results showed that the Chromosome breakage rate per cell （b/e） were significantly higher in BD-exposed workers than that in control group [ （ 1.06± 0. 41 ） % vs （ 0. 85 ±0. 36 ） %, P 〈 0. 01 ], and the levels of b/c were significantly lower in BD-exposed workers with AG＋GG genotype of A245G polymorphism of XRCC4 than those with AA genotype[ （0. 91±0. 29）% vs （ 1.18±0.48）%, P=0. 02], while the levels of b/c[ （ 1.13±0. 42）%] in GG genotype of XRCC4 T1394G polymorphism were significantly higher than that of the Tr genotype [ （0. 75±0. 36）%, P= 0. 04 ]. Conclusion The results suggested that there could be a correlation between polymorphisms of XRCC4 and DRC of DNA damage induced by BD.
出处
《中国工业医学杂志》
CAS
2016年第3期171-173,204,共4页
Chinese Journal of Industrial Medicine
基金
唐山市科技支撑计划项目(No.13130297z)
