摘要
本试验采集病死鸽临床样品,用SPF鸡胚接种传代分离病毒后,通过血凝(HA)与血凝抑制(HI)试验、RT-PCR及F基因部分片段测序与分析对分离株进行鉴定,并通过测定鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)和动物回归试验评估分离株的毒力和致病性。结果表明,从病鸽组织病料中分离获得1株鸽Ⅰ型副黏病毒(PPMV-1),命名为GXP120012。分离株GXP120012的F基因裂解位点112-117位的氨基酸组成为R-R-Q-K-R-F,符合强毒株特征;GXP120012与PPMV-1参考毒株pi/CH/LLN/110713的核苷酸序列同源性高达98.9%,并处于同一遗传分支,基因型为Ⅵ型。该分离株的MDT为102h,ICPI为0。动物回归试验发现,分离株GXP120012对鸽具有较强的致病性,而对鸡没有致病力。本试验结果为今后鸽新城疫的分子流行病学研究和疾病防控提供理论依据。
A pigeon paramyxovirus serotypeⅠ（PPMV-1）isolate was recovered from samples col-lected from dead pigeons and initial isolation of the virus was performed in 9-day-old specific-pathogen-free （SPF）chicken embryos.The isolate was identified by the hemagglutination （HA） test,hemagglutination-inhibition （HI）assays,RT-PCR,sequencing and analysis of partial of F gene of the virus.The virulence and pathogenicity of the isolate were determined by mean death time （MDT）,intracerebral pathogenicity index （ICPI）in 1-day-old chicks and animal regression experiment.The results indicated that one PPMV-1 was isolated from dead pigeon and named as GXP120012.The F gene sequence analysis of PPMV-1 GXP120012 strain showed that the cleav-age sites of the virus was 112 R-R-Q-K-R-F117 motif in accordance with characteristic of velogenic strains;The nucleotide sequence homologies of F gene between strain GXP120012 and PPMV-1 reference strain pi/CH/LLN/110713 were high and reached 98.9%.They located the same branch on phylogenetic tree and fell into genotype Ⅵ.The MDT and ICPI values of isolate were 102 h and 0,respectively.The results of animal regression experiment showed that GXP120012 isolate was virulent for pigeons and avirulent for chickens.The present study provided useful material and theoretical basis for the study of molecular epidemiology and prevention and control of New-castle disease in pigeon in future.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第7期1856-1861,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31360612)
广西自然科学基金项目(2013GXNSFAA019080)
广西科技攻关项目(桂科攻1123007-4)
广西大学生创新创业训练计划项目(201410593118
SYJN20121701)
关键词
鸽Ⅰ型副黏病毒
分离鉴定
致病性
基因型
pigeon paramyxovirus serotype I
isolation and identification
pathogenicity
genotype
