摘要
利用三聚氰胺对乙酰半胱氨酸-三乙醇胺复合包覆的碲化镉量子点荧光的猝灭作用,建立了快速测定尿样中三聚氰胺的荧光传感新技术。研究结果表明,三聚氰胺浓度在0.1-1.0μmol/L范围内,量子点的荧光强度猝灭值（ΔF）与三聚氰胺的浓度呈线性关系,线性方程为ΔF=202.89cMA＋38.26,相关系数（r）为0.9959,方法检出限0.05μmol/L。本方法用于尿液加标样品中三聚氰胺的检测,RSD为2.67%,三聚氰胺的平均回收率为98%-106%。
A new fluorescent sensor method for determination of melamine in urine was developed based on fluorescent quenching function of acetylcysteine-triethauolamine-coated CdTe quantum dots by melamine. Experimental results show that, a good linear relationship between the fluorescent intensity quenching （AF） and the concentration of melamine can be obtained at the range of 0.1-1.0 μmol/L in the buffer solution of pH 8.57 with a detection limit 0.05 μmol/L. The calibration equation is AF= 202.89cMA ＋ 38.26 with a correlation coefficient（r） 0.995 9. The method can be applied in determination of melamine in spiked urine samples with 2.67% RSD, and the spiked recovery of 98%-106%.
出处
《当代化工》
CAS
2016年第6期1145-1147,共3页
Contemporary Chemical Industry
基金
台州市科技计划项目
项目号:131KY08
浙江省科技计划项目
项目号:2014C37052
2015C33224
浙江省应用化学重点学科(台州学院)
台州学院培育基金项目
项目号:2013PY30
关键词
荧光传感
三聚氰胺
CDTE量子点
荧光猝灭
三乙醇胺
Fluorescence sensor
Melamine
CdTe quantum dots
Fluorescence quenching
Triethanolamine
