摘要
目的:探讨补肾方对激素性股骨头坏死(osteonecroois of the femoral head,ONFH)大鼠股骨头血管形态和血液状态的影响。方法:将120只雄性Wistar大鼠随机分为空白组、模型组、健骨生丸组、补肾方高剂量组、补肾方中剂量组和补肾方低剂量组,每组20只。除空白组外,其余5组大鼠臀肌注射甲泼尼龙琥珀酸钠,连续注射3 d,建立激素性ONFH模型。甲泼尼龙琥珀酸钠注射完后,空白组、模型组自由饮食;健骨生丸组按1.68 g·kg^(-1)以健骨生丸灌胃,每天1次,连续6周;补肾方高、中、低剂量组分别按21.2 g·kg^(-1)、10.6 g·kg^(-1)、5.3 g·kg^(-1)以补肾方灌胃,每天1次,连续6周。药物干预结束后,先从各组随机选取10只大鼠,麻醉后腹主动脉取血,动物死亡后,取双侧股骨头制成石蜡切片。将所取腹主动脉血分成2份,一份取血清测定甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL)、载脂蛋白A1(apolipoprotein A1,Apo A1)及Apo B;另一份取全血以旋转法测定全血黏度低切值、中切值和高切值,取血浆检测血浆黏度。制成的股骨头组织切片分成2份,一份进行HE染色,光学显微镜下观察骨组织形态;另一份进行免疫组织化学染色,测定血管内皮生长因子(vascular endothelial growth factor,VEGF)和FLK1蛋白表达情况。各组剩余的10只大鼠应用血管造影结合Micro-CT扫描技术测定股骨头中血管体积、血管表面积、血管体积分数及血管厚度。结果:1血脂检测结果。6组大鼠血清TG、TC、LDL、HDL、Apo A1和Apo B含量比较,组间差异均有统计学意义(F=4.538,P=0.004;F=3.322,P=0.018;F=2.681,P=0.043;F=2.621,P=0.047;F=2.400,P=0.035;F=3.741,P=0.010)。空白组、健骨生丸组、补肾方中剂量组和补肾方高剂量组TG、TC、LDL、Apo B含量均低于模型组(P=0.000,P=0.021,P=0.009,P=0.032;P=0.008,P=0.016,P=0.031,P=0.030
Objective：To explore the effect of Bushen Fang（补肾方, BSF）on blood vessel nlorphous of femoral head and blood state in rats with steroid-imtneed necrosis of femoral head（SNFH）. Methods：One hundred and twenty Wistar ruale rats were randondy divided into blank group, model group, Jiangusheng Wan （健骨生丸 JGSW）group, BSF high-dose group, BSF middle-dose group and BSF low-dose group ,20 cases in each group. The rats in model group, JGSW group, BSF high-dose group, BSF middle-dose group arid BSF low-dose group were treated with intra-gluteal injection of methylprednisolone sodium succinate for consecutive 3 days to build the SNFH models. Then the rats in blank group aud model group were permitted to drink and eat freely. The rats in JGSW group were intragastric administrated with J（,- SW（ 1.68 g/kg） ,once a day tot consecutive 6 weeks;while the others in BSF high-dose group, BSF middle-dose group and BSF low-dose group were intragastric administrated with BSF（21.2,10.6 and 5.3 g/kg,respectively）, once a day for consecutive 6 weeks. After the end of drug intervention, 10 rats were randomly selected from each group,and their blood were fetched out from aorta abdominalis after anesthe- sia and the bilateral temoral heads were fetched out lot making paraffin sections after the rats died. The blood were divided into 2 parts,and one of them were applied to measure the serum contents of triglyceride （TG）, total cholesterol （TC）, high density lipoprotein （ HDL）, low density lipoprotein （LDL）, apolipoprotein A1 （ApoA1）and ApoB ;while the other portion were applied to measure the high, medium and low shear whole blood viscosity and the plasma viscosity. The acquired tissue sections of femoral head were divided into 2 parts, and one of them were received HE staining for observing the bone tissue morphology under optical microscope, while the other portion were received immuno- histochemical staining for detecting the expression of vascular endothelial growth factor（VEGF
出处
《中医正骨》
2016年第6期1-11,共11页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
国家自然科学基金项目(81173417
81373656)
关键词
股骨头坏死
大鼠
Wistar
补肾方
血管形态
血液状态
动物实验
femur head necrosis
rats, wistar
Bushen Fang
morphous of blood vessel
state of blood
animal experimentation
