摘要
目的建立并比较河北省不同产地葎草Humulus scandens的HPLC特征图谱。方法色谱柱为Agilent 5 TC-C18(250mm×4.6 mm,5μm);流动相为乙腈-0.2%磷酸水溶液;体积流量1.0 m L/min;检测波长340 nm;柱温25℃;进样量10μL。收集河北省不同产地11批葎草药材进行测定,建立11批药材的特征图谱,并利用《中药色谱指纹图谱相似度评价系统(2004版)》对11批样品的相似度进行评价。结果从葎草药材特征图谱中分析得出9个共有峰,其中7号峰为木犀草苷,8号峰为芹菜素-7-O-β-D-葡萄糖苷。经分析,其中7批药材的相似度0.900以上,4批药材的相似度在0.900以下。结论该方法简便、准确、重复性好,可为有效控制葎草药材的内在质量标准提供科学依据。
Objective To establish and compare the HPLC characteristic spectra of Humuli Scandentis Herba form different habitats in Hebei province. Methods HPLC was performed on Agilent 5 TC-C18（250 mm × 4.6 mm, 5 μm） column, with the mobile phase of acetonitrile-0.2% phosphoric acid at flow rate of 1.0 m L/min; Detection wavelength was 340 nm; The column temperature was 25 ℃and the sample size was 10 μL. Eleven batches of Humuli Scandentis Herba samples form different habitats in Hebei province were determined and the characteristic spectra of those were established. The fingerprint evaluation software（2004 edition） for Chinese materia medica（CMM） was used to evaluate the similarity of the 11 batches of samples. Results There were nine characteristic peaks identified in the characteristic spectra of Humuli Scandentis Herba samples. Peak 7 was luteoloside and Peak 8 was apigenin-7-O-β-D-glucosidase. The similarities of seven batches of Humuli Scandentis Herba samples were proved to be higher than 0.900 and four batches of them were proved to be less than 0.900. Conclusion The method is simple, accurate, and reproducible, and can provide the scientific evidence for controlling the internal quality standards effectively.
出处
《中草药》
CAS
CSCD
北大核心
2016年第10期1775-1779,共5页
Chinese Traditional and Herbal Drugs
