摘要
目的:建立降香中木犀草素和异甘草素的含量测定方法。方法:用高效液相色谱法测定了木犀草素和异甘草素的含量,色谱柱:Zorbax eclipse XDB C18(150mm×4.6mm),木犀草素:流动相:甲醇-0.4%磷酸溶液(43:57);流速:1.0mL/min,测定波长:353nm;结果:平均回收率98.9%(RSD=2.6%),线性关系r=0.9997,重复性RSD=1.6%,精密度RSD=1.5%。异甘草素:流动相:甲醇-乙腈-0.025mol/l磷酸(18:20:62)流速:1.0mL/min,测定波长:368nm;结果:线性关系r=0.9998,重复性RSD=1.8%,精密度RSD=1.8%。结论:方法稳定、可靠,可作为其质量控制方法。
Objective:To develop a method for the determination of Luteolin in rosewood.Methods: Determining the Luteolin in Duyiwei Pellet by HPLC.The separation was performed on Zorbax eclipse XDB C18(150mm×4.6mm ) with Meth-0.4%H3PO4(43:57);Meth -Acetonitrile-0.025mol/l H3PO4(18:20:62) as mobile phase and UV detector at 353 nm;368nm.Results:The average recovery was 98.9%(RSD=2.6%), with good linear relationship(r=0.9997)and repetition RSD=1.6% and precision RSD=1.5%.Conclusions:The method is accu—rate,reliable and c...
出处
《辽宁中医药大学学报》
CAS
2009年第3期171-174,共4页
Journal of Liaoning University of Traditional Chinese Medicine
