摘要
目的研究苯并(a)芘[Benzo(a)pyrene,B(a)P]暴露对职业人群尿中1-羟基芘(1-OH-Py)及外周血CYP1A1mRNA水平的影响,探讨两者之间的关系。方法选取118名焦化厂车间作业工人接触组和63名非职业接触人群为对照组进行问卷调查;收集研究对象工作班后6h尿样,晨起空腹抽取静脉血。用高效液相色谱法-荧光检测器法检测尿中1-OH-Py的含量,QT-PCR检测外周血细胞中CYP1A1 mRNA水平。结果接触组1-OH-Py为(0.623±0.762)μmol/mol Cr,对照组为(0.272±0.231)μmol/mol Cr,差异有统计学意义(P<0.05)。接触组CYP1A1水平为0.309±0.552,对照组为0.479±0.586,差异有统计学意义(P<0.05)。尿1-OH-Py水平与外周血CYP1A1的基因表达水平之间呈负相关(r=-0.219,P=0.017)。结论尿中1-OH-Py的水平可以作为职业暴露人群的外暴露生物标志物,接触B(a)P的职业人群外周血CYP1A1 mRNA表达水平降低,且两者之间有相关性。
Objective To study the effects of occupational exposure to benzo（a）pyrene on 1-hydroxy pyrene（1-OH-Py）level in urine and CYP1A1 mRNA expression level in peripheral blood. Methods A questionnaire survey was performed to118 coking plant workshop workers（as a contact group） and 63 water supply workers（as a control group）, and their urinesamples after work 6 hours and morning fasting elbow venous blood samples were collected. The urine 1-OH-Py level wasdetected by the high performance liquid chromatography（HPLC） method, and the mRNA of CYP1A1 expression level inperipheral blood cells was detected by QT-PCR. Results The mean levels of 1-OH-Py were（0.623±0.762） μmol/mol Cr inthe contact group and（0.272±0.231） μmol/mol Cr in the control group respectively, and there was a significant differencebetween the two groups（P〈0.05）. The levels of CYP1A1 were（0.309±0.552） in the contact group and（0.479±0.586） in thecontrol group respectively, and there was a significant difference between the two groups（P〈0.05）. The urine 1-OH-Py leveland CYP1A1 gene expression of peripheral blood cells showed a negative correlation（r =-0.219, P = 0.017）. Conclusion The 1-OH-Py level in urine can be used as a biomarker of outside to occupational exposure population, and the peripheralblood CYP1A1 mRNA expression level of the contact benzo（a）pyrene occupational population is reduced, and they are relatedwith each other.
出处
《中国热带医学》
CAS
2016年第6期612-614,共3页
China Tropical Medicine
基金
国家自然科学基金资助项目(No.81001285)
太原钢铁集团卫生处资助项目(No.2014)
