摘要
目的探讨持续性与周期性流体静压力对骨髓间充质干细胞（BMSCs）软骨向分化潜能的影响。方法采用全骨髓贴壁法分离培养大鼠BMSCs,并采用成骨成脂分化鉴定。细胞随机分为5组,分别为对照组,持续性静压力45 k Pa、90 k Pa组,周期性动压力0~45 k Pa、0~90 k Pa作用组,压力刺激组细胞采用每天1 h连续2 d的加载方式。采用Real-time PCR对细胞成软骨分化标志基因Sox9、Aggrecan及Col-Ⅱ表达水平进行检测。结果 45 k Pa、90 k Pa的静压力及0~45 k Pa、0~90k Pa动压力作用下,BMSCs中Sox9的基因表达量显著高于对照组（P〈0.05）;45 k Pa,90 k Pa,0~90 k Pa组中Aggrecan mRNA的表达量明显升高（P〈0.01）;90 k Pa,0~90 k Pa压力作用组中Col-Ⅱ的表达量显著高于对照（P〈0.01）。结论压力微环境刺激可明显促进BMSCs成软骨标志基因的表达,且持续90 k Pa的压力作用下BMSCs成软骨分化效果显著。
Objective To investigate the different effects of continuous and cyclic hydrodynamic pressure on marker gene expression of chondrogenic differentiation of bone marrow mesenchymal stem cells （BMSCs）. Methods BMSCs were obtained by whole bone marrow adherent separation culture method and identified by osteogenic and adipogenic induction assays. BMSCs were randomly divided into a control group and four experimental groups which were treated by continuous hydrostatic pressure at 45kPa or 90kPa, and cyclic hydrostatic pressure at 0-45kPa or 0- 90kPa. The experimental groups were treated with pressure lh per day for 2 days. The expression of Sox9, Aggrecan and Col-Ⅱ was detected by Real-time PCR. Results Real-time PCR showed that under pressure, the expression of Sox9 was significantly enhanced compared to that in the control group （P〈0.05）. The expression of Aggrecan in the cells under 45, 90 and 0- 90kPa was significant higher than that of control group （P〈0.01）. The expression of Col-Ⅱ gene was significantly increased un- der 90 and 0-90 kPa （P〈0.01）. Conclusion Hydrodynamic pressure could promote the chondrogenic differentiation of BMSCs. The effect of 90 kPa on the chondrogenic differentiation is remarkable.
出处
《口腔医学》
CAS
2016年第6期481-484,共4页
Stomatology
基金
国家自然科学基金资助项目(81371188,31570951)
关键词
流体压力
骨髓间充质干细胞
成软骨分化
hydrodynamic pressure
bone marrow mesenchymal stem cells （BMSCs）
chondrogenic differentiation
