摘要
目的通过比较溶血磷脂酸受体-3（LPAR3）在小鼠胚胎着床期子宫内膜的表达,了解坤泰胶囊对小鼠子宫内膜容受性的影响。方法将90只6-8周龄的昆明雌鼠分为3组[A组：控制性超促排卵（COH）;B组：坤泰＋COH;C组：生理盐水对照）,每组30只],检查合笼次日晨阴栓,于第1-6天每天每组处死5只雌鼠,测定血清中雌二醇（E2）、孕酮（P）水平,以及腺体数和LPAR3免疫组化光密度值（IOD）。结果 A、B组血清E2、P水平值均高于同期C组（P〈0.05）;HE染色,A组较B、C组腺体数明显减少（P〈0.05）。A组第3天LPAR3的IOD值高于B、C组（P〈0.05）,而A组第4天IOD值明显小于B、C组（P〈0.05）,B组较C组略高,但差异无统计学意义（P〉0.05）。结论坤泰胶囊可能通过改善COH小鼠LPAR3正常时空表达,起到改善子宫内膜容受性的作用。
Objective To understand the effect of Kuntai Capsule on mice endometrial receptivity by comparing the lyso‐phospholipids acid receptor‐3（LPAR3） expression in endometrium during mouse embryos implantation period .Methods Ninety 6-8 week old Kunming female mice were randomly divided to three groups ：the group A [controlled ovarian hyperstimulation （COH）] ,B （COH＋Kuntai Capsule） and C（normal saline control） ,30 cases in each group .The vaginal plug was performed on the next day after mating .Five mice in each group were daily killed on 1 -6 d .The serum estradiol（E2） and progesterone（P） levels were detected .The glands number and the immunohistochemical integrated optical density （IOD） were also determined .Results The levels of serum E2 amd P in the group A and B were higher than those in the group C at the same period （P〈0 .05）;in the HE staining ,the glands number in the group A was significantly decreased compared with the group B and C （P〈0 .05） .The IOD value of LPAR3 on 3 d in the group A was significantly lower than that in the group B and C （P〈0 .05） ,while the group B was slightly higher than the group C without statistical difference （P〉0 .05） .Conclusion Kuntai Capsule may play the role for improving the endometrial receptivity by improving the normal space‐time expression of LPAR3 .
出处
《重庆医学》
CAS
北大核心
2016年第18期2464-2466,共3页
Chongqing medicine
基金
2012年河南省高等学校骨干教师资助项目(2012GGJS-135)
