摘要
目的评价VITEK-2Compact细菌自动化鉴定与药敏仪器对碳青霉烯类耐药肠杆菌科细菌(CRE)的鉴定能力。方法同时采用PCR法和VITEK-2Compact系统检测本实验室保存的93株CRE。以PCR结果为标准,评价VITEK-2Compact仪器对CRE菌株的鉴定能力。结果 VITEK-2Compact细菌自动化仪器检测CRE的灵敏度为76.4%、特异性为34.2%、阳性预测值为62.7%、阴性预测值为50.0%。结论VITEK-2Compact细菌自动化仪器检测CRE的灵敏度有待提高,需结合其他方法给予补充,以提高该仪器的鉴定率与准确率。
Objective To evaluate the performance of VITEK-2Compact Automated Systems for the detection of carbapenemase-resistant Enterobacteriaceae（CRE）. Methods 93 CRE stored in our laboratory were used in this study. The bacterial identification and antibiotic susceptibility tests were performed by VITEK-2Compact automated system. Some common carbapenem-resistant genes were amplified by PCR,and the positive PCR products were sequenced to determine genotypes. With the PCR results as the standard,we evaluated the performance of VITEK-2Compact Automated Systems for the detection of carbapenemase-resistant Enterobacteriaceae. Results The sensitivity,specificity,positive predictive value,and negative predictive value of VITEK-2 Compact to detection CRE were 76.4%,34.2%,62.7%,and 50.0%,respectively. Conclusion The sensitivity of the VITEK-2Compact Automated Systems for detection of CRE needs to be improved. In order to enhance the identification rate and accuracy of the instrument,the laboratory should complement other methods.
出处
《福建医科大学学报》
北大核心
2016年第2期133-135,共3页
Journal of Fujian Medical University
基金
国家自然科学基金(81201328)
福建省高校杰青项目(JA13134)
福建省卫计委中青年骨干人才培养项目(2015-ZQN-ZD-15)
关键词
微生物敏感性试验
药物耐受性
细菌
肠杆菌科
青霉属
抗菌药
专家系统
microbial sensitivity tests
drug tolerance
bacteria
enterobacteriaceae
penicillium
anti-bacterial agents
expert systems
