摘要
目的：克隆红花中查耳酮异构酶（Chalcone-flavonone isomerase,CHI）基因,进行生物信息学分析、开花1~7 d CHI表达量及羟基红花黄色素A（hydroxysafflor yellow A,HSYA）含量分析,为红花中CHI功能验证、黄酮类成分合成及调控机制研究提供基础。方法：克隆CHI序列,通过生物信息学预测CHI特性,使用Real-time PCR检测红花开花1~7 d CHI的相对表达量,HPLC检测HSYA动态累积的百分含量,并进行关联分析。结果：克隆获得红花CHI序列全长,检测得到红花开花1~7 d CHI的相对表达量及HSYA动态累积的百分含量。CHI表达量及HSYA动态累积百分含量呈现相似的变化趋势：均在1~4 d逐渐升高,在第4天达到最高峰,第5~7天急剧降低。结论：此研究可为红花中CHI功能验证、黄酮类成分合成及调控机制研究提供基础。
Objective： To clone chalcone-flavonone isomerase（ CHI） gene in Carthamus tinctorius,to analyze the bioinformation of CHI,to compare the expression of CHI,and to analyze the percentage composition of hydroxysafflor yellow A（ HSYA） during the florescence,in order to provide the foundation for functional verification of CHI and the composition and regulation mechanism of the flavonoid constituents in Carthamus tinctorius. Methods： CHI was cloned,bioinformatics was used to analyze the protein characteristics,real timePCR was used to analyze the expression of CHI,and HPLC was used to analyze the percentage composition of HSYA. Results： A 696 bp CHI sequence in Carthamus tinctorius,the expression of CHI and the percentage composition of HSYA during the florescence were obtained. The variation tendency was similar between the expression of CHI and the accumulation of HSYA,which was increased gradually during 1 ~ 4 d and peaked at the fourth day,then decreased sharply during the fifth to the seventh day of florescence. Conclusion： This research provides a foundation for functional verification of CHI and the composition and regulation mechanism of the flavonoid constituents in Carthamus tinctorius.
出处
《中药材》
CAS
CSCD
北大核心
2016年第3期499-503,共5页
Journal of Chinese Medicinal Materials
关键词
红花
查耳酮异构酶
基因克隆
生物信息学分析
基因表达
羟基红花黄色素A
Carthamus tinctorius L Chalcone-flavonone isomerase Gene clone Bioinformations analysis Gene expression Hydroxysafflor yellow A
