摘要
目的建立椰子汁饮料中椰醛的气相色谱分析方法。方法椰子汁样品经亚铁氰化钾-乙酸锌沉淀蛋白,无水乙醚萃取,无水硫酸钠干燥后经HP-1701石英毛细管色谱柱(30 m×0.25 mm,0.25μm)分离,氢火焰离子化检测器(FID)检测,以保留时间定性,峰面积定量,采用外标标准曲线法定量。结果椰醛在浓度为0μg/ml^50.0μg/ml时,线性关系良好,回归方程为y=0.786x+0.580,线性相关系数(r)为0.999,椰子汁饮料中椰醛的检出限和定量限分别为0.30 mg/kg和1.0 mg/kg。在2.0 mg/kg、5.0 mg/kg、10.0 mg/kg 3种添加水平下,饮料中椰醛的加标回收率为78.4%~86.8%,相对标准偏差(RSD)为5.01%~6.19%(n=6)。结论本方法操作简单、灵敏度高、检出限低、稳定性好,适合饮料中椰醛的测定。
Objective To develop a method for the determination of nonanolactone in coconut milk by gas chromatographic method. Methods Coconut milk samples were purified by potassium ferrocyanide-zinc acetate for protein precipitation,extracted by ether,and dried with sodium sulfate,and then separated on HP-1701（ 30 m × 0. 25 mm,0. 25 μm） capillary column,and detected by FID. The retention time and the peaks area were used for identification and quantification respectively.The external standard method was used for quantitative analysis. Results The method had a good linearity when the concentration of coconut aldehyde was among 0 μg / ml-50. 0 μg / ml with the regression equation of y = 0. 786 x ＋ 0. 580,and the lineaer coefficient（ r） of 0. 999. The limit of detection and limit of qualification of the method was 0. 30 mg / kg and 1. 0 mg / kg,respectively. The average recoveries were in the range of 78. 4 %-86. 8% at the spiked levels of 2. 0 mg / kg,5. 0 mg / kg and 10. 0mg / kg; and the relative standard deviation（ RSD） were among 5. 01%-6. 19%（ n = 6）. Conclusion The method is simple,rapid,sensitive,stable,and is suitable for the determination of nonanolactone in coconut milk.
出处
《中国卫生检验杂志》
CAS
2016年第11期1546-1547,共2页
Chinese Journal of Health Laboratory Technology
关键词
椰醛
椰汁饮料
蛋白沉淀
气相色谱法
Nonanolactone
Coconut milk
Protein precipitation
Gas chromatography
