摘要
目的:探讨不同造模时间下大鼠滑膜组织中的Toll样受体(Toll-like Receptors,TLRs)和核因子-kB(NF-kB)在骨关节炎(Osteoarthritis,OA)病程中的内在联系,研究其表达特点及变化规律。方法:将80只Wistar大鼠随机分为5组,每组16只。模型组大鼠利用经典Hulth法建立2,4,6周膝OA动物模型,同样方式切开假手术组皮肤及关节囊进入关节腔,空白对照组不予手术。按期提取滑膜样本,对其进行形态学观察并用实时PCR法检测各组滑膜组织中TLR2,TLR4及NF-kB的表达。结果:重度OA模型中的TLR2表达显著增加(P<0.05);随着造模时间的延长,TLR4的表达逐渐受到抑制(P<0.05);NF-kB在2,4,6周模型组中均大量表达(P<0.05),且表达量与造模时间成正比。结论:大鼠膝关节OA模型造模成功,OA病程时间与TLR2,TLR4和NF-kB的表达之间存在显著的相关性,三者是导致OA发生的重要影响因子。
Objective：To investigate internal influence of the expression of Toll-like receptors（TLRs）and nuclear factorkB（NF-kB）in the synovial tissue of rats at different modeling time points in the process of osteoarthritis（OA）,as well as to explore the expression features and the change rules.Methods：All 80 Wistar rats were randomly divided into five groups,16 rats in each group.Surgery Groups（knee OA model of 2weeks,4weeks,and 6weeks）were intervened by classical Hulth methods.For the sham operation group was only exposed the knee joint cavity,while no surgery was applied in the blank control group.The samples of knee osteoarhritic synovial tissue were extracted at 2weeks,4weeks and6 weeks after the surgery respectively.The morphology of synovial tissue was observed,and the expression of TLR2,TLR4,and NF-kB of synovial tissue were detected by Real-time PCR methods.Results：The expression of TLR2 in the severe OA group were increased significantly（P〈0.05）.The expression of TLR4 was inhibited gradually with the extension of modeling time（P〈0.05）.The expression of NF-kB was abundantly expressed in the mild,moderate,and severe grade model group（P〈0.05）,and the expression amount was proportional to modeling time.Conclusion：Rat OA models are effectively produced.The expression of TLR2,TLR4,and NF-kB are related to the disease duration of OA,which are key factors to OA progression.
出处
《中国中医骨伤科杂志》
CAS
2016年第6期4-8,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金面上项目(81373662)
北京中医药大学自主选题项目(2014-JYBZZ-XS-171)
