摘要
目的探讨微小RNA(microRNA,miRNA)miR-21和miR-34在胃癌组织(T)和癌旁正常胃黏膜组织(N)中的表达及其临床意义。方法运用实时荧光定量多聚核苷酸链式反应(PCR)测定T和N中的miR-21、miR-34表达水平。结果(1)T中miR-21、miR-34的表达量分别为(8.32±4.48)和(3.40±0.49),N中两者表达量分别为(4.13±1.29)和(3.86±0.51),T中miR-21的水平明显高于N,miR-34的水平明显低于N,差异有统计学意义(P<0.05)。(2)T中miR-21水平与TNM分期、淋巴结转移有关(P<0.05);T中miR-34表达量与肿瘤大小、组织学分级、TNM分期、淋巴结转移有关(P<0.05)。结论胃癌组织中miR-21的升高和miR-34的下降在胃癌发生、发展中起着重要作用,提示肿瘤的淋巴结转移、侵袭性、远处转移增强。
Objective To investigate the expression of micro RNA（microRNA,miRNA）miR-34and-21 in gastric tumor（T）and adjacent normal gastric mucosa（N）and clinical significance.Methods MiR-21 and miR-34 levels in T and N were measured by real-time fluorescence quantitative PCR.Results（1）The levels of miR-21 in T and N were（8.32±4.48）and（4.13±1.29）（P〈 0.05）and the levels of miR-34 in T and N were（3.40±0.49）and（3.86±0.51）（P〈 0.05）;（2）The level of miR-21 in T was related to TNM stage and lymphatic metastasis（P〈 0.05）,while the level of miR-34 in T was related to tumor size,histological grade,TNM stage,lymphatic metastasis（P〈 0.05）.Conclusion The increase of miR-21 and the decrease of miR-34 in gastric tumor played an important role in the occurrence and development of gastric tumor,which were suggested that the lymphatic metastasis,invasion and distant metastasis of the tumor were enhanced.
出处
《新疆医科大学学报》
CAS
2016年第7期854-857,共4页
Journal of Xinjiang Medical University
基金
新疆医科大学研究生科研创新项目(XJGR12013080)
