摘要
目的:观察癫痫清颗粒对戊四唑点燃癫痫大鼠行为学及脑内NMDAR2B和c-fos表达的影响,探讨其抗癫痫的作用机理。方法:腹腔注射戊四唑建立癫痫大鼠点燃模型,连续灌胃给药30 d后,观察癫痫清颗粒对大鼠行为学及脑组织NMDAR2B和c-fos的影响。结果:癫痫清颗粒可延长癫痫大鼠惊厥潜伏期,缩短惊厥持续时间,减少组织NMDAR2B和c-fos的表达。结论:癫痫清颗粒对戊四唑点燃大鼠模型具有抗癫痫作用,作用机制与减少脑内NMDAR2B和c-fos的表达有关。
Objective： To study the anti-epileptic mechanism of Dianxianqing Granule by observing the effects on behavior,NMDAR2 B and c-fos expression in brain of epilepsy model rats kindled by pentylenetetrazol（ PTZ）. Methods：Kindling models were established by intraperitoneal injection of PTZ on rats,intragastric administration for 30 days,continuously. The expressions of NMDAR2 B and c-fos in the hippocampus of rats were measured with immunohistochemistry assay. Results： Dianxianqing Granule can prolong the latency of seizure,shorten the duration of seizure and reduce the expressions of NMDAR2 B and c-fos in brain. Conclusion： Dianxianqing Granule has anti-epileptic effect on PTZ kindling epileptic model rats through inhibition of NMDAR2 B and c-fos expression.
出处
《中华中医药学刊》
CAS
北大核心
2016年第6期1319-1322,I0003,I0004,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家"十二五""重大新药创制"专项项目(2012ZX09102201-005)
