摘要
目的:分析金葡球菌和肺炎链球菌在体内对莫西沙星的耐药性。方法:采用大鼠肉芽肿袋模型,体内试验选用野生株、第一代耐药株以及多代耐药株,使用不同菌株感染大鼠产生肉芽肿袋后分为A、B、C3个组,用莫西沙星进行不同剂量用法治疗,每天收集肉芽肿袋的渗出物,根据耐药株的产生测定金葡球菌与肺炎链球菌对莫西沙星的耐药性。结果:由于莫西沙星对试验菌株与大鼠肉芽肿袋中分离菌的MIC相同,所以敏感性无显著变化。两种感染病原体A组与B组的试验结果相同,A组治疗1d后肺炎链球菌减少约107CFU·m L^(-1),A组治疗1d后金葡球菌的减少数量较慢,3d后减少数量明显。B组的肺炎链球菌与金葡球菌的生存率都迅速下降,但减少幅度差异不明显,莫西沙星对细菌生存数量的影响可持续6d,影响期间细菌没有发生再生长,莫西沙星对肺炎链球菌的杀菌能力高于对金葡球菌的杀菌能力。同时B组与C组对比,50mg·kg^(-1)·d^(-1)的莫西沙星杀菌速度和程度都会明显降低,但杀菌效果仍在,治疗1d后细菌可降低103CFU·m L^(-1),效果持续4d,但50mg·kg^(-1)·d^(-1)的莫西沙星、感染后24h治疗对多代耐药株的疗效几乎为零。结论:即使人体内莫西沙星的浓度低于预期的治疗浓度,也对金葡球菌与肺炎链球菌的野生株与第一代耐药株具有良好的杀菌效果。
Objective： Staphylococcus auras and Streptococcus pneumatic in the tamoxifen resistance in vino. Methods： Rats bags granular,wild strains were selected and in vino,as well as the first generation of drug-resistant strains of drug-resistant strains of many generations after using different strains of rats infected produce granules bags into A,B,C3 groups,with different doses of tamoxifen therapy usage,gather daily granular pouch exude was measured Staphylococcus auras and Streptococcus pneumatic resistance to tamoxifen according resistant strains. Results： Since tamoxifen rat granular pouch test strain isolates MIC same,so no significant change sensitivity. Both pathogens A group with the test results the same group B,group A after treatment 1d by about 107CFU·m L-1Streptococcus pneumatic,group A after treatment 1d reduce the number of Staphylococcus aureus slow,3d after reducing significantly the number. Group B Streptococcus pneumatic and Staphylococcus auras survival are rapidly decreased,but the decrease was not significantly different amplitude,the bacteria did not occur during the re-growth of bacteria affect the survival of tamoxifen sustainable6 d, influence, and tamoxifen Streptococcus pneumatic is higher than for bactericidal capacity Staphylococcus auras bactericidal capacity. While group B and group C contrast,50mg·kg-1·d-1 tamoxifen sterilization speed and extent will significantly reduce,but still bactericidal effect,bacteria can reduce treatment 1d days 103CFU·m L-1,the effect lasts 4 days,but 50mg·kg-1·d-1 of tamoxifen,24 h post-infection therapy on multiple generations of resistant strains is almost zero. Conclusion： Even if the body Modishness stars concentrations below the expected therapeutic concentrations,still wild strains of Staphylococcus auras and Streptococcus pneumatic and the first generation of drug-resistant strains has a good bactericidal effect.
出处
《北方药学》
2016年第6期168-169,共2页
Journal of North Pharmacy
关键词
金葡球菌
肺炎链球菌
莫西沙星
耐药性
Staphylococcus auras
Streptococcus pneumatic
Tamoxifen
Resistance
