摘要
为了确立红树莓组培苗生长的适宜光质和光强,以红树莓"海尔特兹"组培苗为试材,研究了不同光质和光强处理下红树莓生根组培苗的叶绿素含量及叶绿素荧光参数。结果表明:光质处理中,除R/B(红光∶蓝光=1∶1)处理外,Chl a(叶绿素a)、Chl b(叶绿素b)和Ct(叶绿素总含量)均随着R/B比率的增加呈现先增加后减小的趋势,Chl a和Ct均在2R/B(红光∶蓝光=2∶1)处理下呈现最大值,分别为1.344、1.716mg·g^(-1),Chl b则在R/2B(红光∶蓝光=1∶2)处理最高,为0.423mg·g^(-1),Chl a、Chl b和Ct均以10R/B(红光∶蓝光=10∶1)处理最低,分别为0.130、0.033、0.163mg·g^(-1),均显著低于其它光质;Chl a/b(叶绿素a/b)在10R/B、2R/B和R/B处理下较高,分别为3.942、3.709、3.653,呈现阳生植物的特性,而在6R/B(红光∶蓝光=6∶1)、W(白光)、4R/B(红光∶蓝光=4∶1)等处理下较低,分别为2.729、2.730、2.747,呈现阴生植物的特性;在R/2B处理下,红树莓组培苗叶片的叶绿素荧光参数值表现极优,Fv/Fm(PSⅡ最大光化学效率)值达到0.881,而10R/B处理下,Fv/Fm值降低到0.355,且显著低于其它光质处理;光强处理中,随着光强的增加,Chl a、Chl b和Ct呈现递减的趋势,最大值由1.218、0.503、1.720mg·g^(-1)分别降低到0.907、0.302、1.209mg·g^(-1),均形成显著差异,Chl a/b则表现为随着光强的增加而增加,在2 000lx处理达到最大值,为3.042,且3种光强处理差异显著;叶片的Fo(初始荧光)随着光强的增加而增加,在2 000lx处理下达到最大值,为180.000,Fm(最大荧光值)则在1 500lx处理下达到最大值,为907.000,而叶片的Fv/Fm、Fv/Fo(PSⅡ的潜在活性)、ΦPSⅡ(PSⅡ实际光化学效率)、Rfd(可变荧光下降比值)则均随着光强的增加呈现递减的趋势,均在1 000lx处理下呈现最大值,分别为0.847、5.573、0.815、4.200。
In order to establish suitable light quality and light intensity for the growth of red raspberry tissue culture plantlets,using red raspberry‘Heritage＇tissue culture plantlets as experimental material,red raspberry root chlorophyll content and chlorophyll fluorescence parameters of tissue culture plantlets of different light quality and light intensity were studied.The results showed that,chlorophyll a,chlorophyll b and the total chlorophyll content increased first and then decreased with increasing of R/B ratio except R/B treatment,chlorophyll a and the total chlorophyll content showed a maximum value in the treatment of 2R/B,respectively 1.344mg·g（-1）,1.716mg·g（-1）,chlorophyll b was the highest in R/2B,was 0.423mg·g（-1）,chlorophyll a,chlorophyll b and the total chlorophyll content（Ct）were the lowest in 10R/B,respectively 0.130mg·g（-1）,0.033mg·g（-1）,0.163mg·g（-1）,were significantly lower than other light;chlorophyll a/b were higher in 10R/B,2R/B and R/B,were 3.942,3.709,3.653,showing the characteristics of sun plants,and in 6R/B,W and 4R/B were lower,were 2.729,2.730,2.747,and showing the characteristics of shade plants;under R/2B,the chlorophyll fluorescence parameters of raspberry plantlets leaves showed the excellent value,the Fv/Fm value reached0.881,however 10R/B,Fv/Fm decreased to 0.355,and significantly lower than the other light treatment.In light intensity treatment,chlorophyll a,chlorophyll b and the total chlorophyll content showed a decreasing trend with the increase of light intensity,respectively dropped to 0.907mg·g（-1）,0.302mg·g（-1）,1.209mg·g（-1） from the maximum value of 1.218mg·g（-1）,0.503mg·g（-1）,1.720mg·g（-1）,the formation of significant differences,chlorophyll a/b was increased with the increase of light intensity,in the 2 000 lx reached the maximum value,was 3.042,and three kinds of light intensity were significantly different;the initial fluorescence Fo of leaves increased with the increase of light intensity,in the light
出处
《北方园艺》
CAS
北大核心
2016年第11期19-23,共5页
Northern Horticulture
基金
河北省"十二五"科技支撑资助项目(16226806D)
国家现代农业科技成果惠民科技示范工程资助项目(Z141100002314009)
关键词
光质
光强
红树莓
叶绿素荧光
light quality
light intensity
red raspberry
chlorophyll fluorescence
