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卒中后抑郁大鼠情感障碍相关脑区小胶质细胞的增殖变化 被引量:15

The proliferation of microglia in the emotional disorders-related brain regions of rats with post-stroke depression
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摘要 目的 探讨卒中后抑郁(post stroke depression,PSD)大鼠额叶、海马及杏仁核小胶质细胞的增殖表达变化,了解小胶质细胞在PSD发病过程中的作用.方法 将健康成年雌性SD大鼠分为4组:正常组、抑郁组、卒中组及PSD组,每组5只大鼠.抑郁组采用慢性不可预见的中等应激刺激(Chronic unpredictable mild stress,CUMS)结合孤养法建立大鼠抑郁模型;卒中组利用线栓法建立大鼠脑缺血模型;PSD组采用线栓法建立脑缺血模型后,再加以CUMS及孤养法建立PSD大鼠模型.造模后对各组大鼠进行蔗糖水消耗实验和旷场实验检测,于造模后第8周末,应用免疫荧光标记技术检测各组大鼠额叶、海马及杏仁核OX42阳性小胶质细胞的增殖表达变化.结果 在CUMS后第29天,PSD组蔗糖溶液消耗量[(23.8±0.8)%]、大鼠旷场实验水平运动距离[(63.0±1.2) cm]及垂直距离[(25.0±1.0)cm]与正常组[(31.2±1.9)%;(69.8±2.3)cm;(31.0±1.6) cm]、卒中组[(31.0±1.4)%;(70.2±2.4) cm;(30.8±1.1)cm]相比均明显降低,差异有统计学意义(P<0.05).PSD组大鼠额叶、海马、杏仁核OX42阳性细胞数[(20.8±2.6)个;(20.2±1.3)个;(19.8±2.6)个]与正常组[(7.4±2.3)个;(8.0±1.6)个;(9.4±2.1)个]、抑郁组[(8.0±2.0)个;(7.8±2.2)个;(9.2±1.9)个]、卒中组[(9.6±1.1)个;(9.4±2.2)个;(10.2±2.6)个]相比均显著增多,差异有统计学意义(P<0.05).结论 卒中后抑郁大鼠情感障碍相关脑区额叶、海马及杏仁核小胶质细胞数量明显增多,情感障碍相关脑区小胶质细胞的表达增多在PSD发病过程中具有重要意义. Objective To explore the proliferation of microglia in the frontal cortex,hippocampus and amygdala of the post-stroke depression (PSD) in rats.To understand the role of microglia in the pathogenesis of PSD.Methods The adult female SD rats were divided into four groups(n=5 per group):normal control group,depression group,stroke group and PSD group.In the depression group,the depression model rat were established by chronic unpredictable mild stress (CUMS) combined with separately breeding.In the stroke group,focal cerebral ischemic rat models were established with thread embolization method.In the PSD group,focal cerebral ischemic models rat were established with thread embolization method firstly,and then PSD models rat were established with comprehensive chronic unpredictable mild stress(CUMS) and separately breeding on this basis.After the procedure,rats were subjected to sucrose preference test and open field test.At the postoperative eighth weekend,immunofluorescence technology was used to detect the proliferation changes of OX42 positive cells in the frontal lobe,hippocampus and amygdale.Results At the 29th day after CUMS,the sucrose solution consumption ((23.8±0.8) %),horizontal movement distance of open field test ((63.0± 1.2) cm) and vertical movement distance ((25.0± 1.0) cm) in PSD group were significantly lower than those in normal group((31.2± 1.9) %;(69.8±2.3) cm;(31.0± 1.6) cm) and depression group((31.0±1.4) %;(70.2±2.4) cm;(30.8± 1.1) cm) (P〈0.05).The number of OX42 positive cells of frontal lobe,hippocampus and amygdale in PSD group((20.8±2.6);(20.2±1.3);(19.8±2.6))increased significantly compared with those of normal group((7.4±2.3);(8.0± 1.6);(9.4±2.1)),depression group((8.0±2.0);(7.8 ±2.2);(9.2±1.9))and stroke group((9.6±1.1);(9.4±2.2);(10.2±2.6)) (all P〈0.05).Conclusion The number of microglia in PSD group in the emotional d
出处 《中华行为医学与脑科学杂志》 CAS CSCD 北大核心 2016年第5期410-415,共6页 Chinese Journal of Behavioral Medicine and Brain Science
基金 国家自然科学基金项目(81360208)National Natural Science Foundation of China
关键词 脑卒中后抑郁 小胶质细胞 额叶 海马 杏仁核 Post-stroke depression Microglia Frontal lobe Hippocampus Amygdala
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