摘要
目的:比较MTA、Biodentine、Endosequence根管封闭剂的生物相容性。方法:选取4只成年雄性SD大鼠,常规麻醉,无菌操作下在大鼠颅骨上制备4个1 mm×1 mm的骨缺损,将MTA、Biodentine、Endosequence根管封闭剂分别埋植于3个骨缺损内,另1个骨缺损为空白对照。于术后第2、4、8、12周各处死1只大鼠,取颅骨做硬组织包埋、切片。VG氏染色后显微镜下观察植入材料的骨组织相容性。结果:术后第2、4周,MTA、Biodentine组可见纤维结缔组织移行,第8周起有新骨生成,并在材料内部不规则生长;第12周,新生骨质完全覆盖于材料表面。术后第2、4周,Endosequence组可见纤维结缔组织逐渐包绕材料;第8周,部分新骨包绕分割材料,并向材料内部不规则生长;第12周,材料表面被新生骨完全覆盖,且有新骨长入材料内部。结论:Biodentine、Endosequence、MTA均具有较好的生物相容性,并表现出一定的骨诱导能力。
AIM： To compare the biocompatibility of MTA,Biodentine and Endosequence root repair materials. METHODS： 4 bone defects in the size of 1 × 1 mm in each skul of 4 SD rats weighted about 300 g were formed by bound bur. The defects were filled with MTA,Biodentine and Endosequence respectively. One of the defects was not filled as the blank control. After 2,4,8 and 12 w of surgery,the rats were respectively sacrificed and the bone was sectioned for histological observation. RESULTS： In MTA and Biodentine groups,bone defects were characterized with fibrous connective tissue migration 2 and 4 w after operation,new bone formation was observed on week 8. On week 12,new bone completely covered the bone implant surface. In Endosequence group,fibrous connective tissue gradually surrounded the material on week 2 and 4. On week 8,new bone was be found abound the filling material and irregular bone growth into the the material was observed. On week 12,new bone completely covered the surface of the filling material and grew into the material. CONCLYSION： MTA,Biodentine,and Endosequence have good biocompatibility in the rat skull bone tissue with osteogenesis induction potential.
出处
《牙体牙髓牙周病学杂志》
CAS
2016年第6期369-372,331,共5页
Chinese Journal of Conservative Dentistry
