摘要
目的:研究不同剂量的白藜芦醇对大鼠海马神经元氧化损伤的影响。方法:通过体外原代培养获得大鼠海马神经元。实验分为对照(control,Con)组、三甲基氯化锡(trimethyltin chloride,TMT)组、溶剂对照(vehicle)组和白藜芦醇(resveratrol,Res)组。其中,Res设4个浓度:5μmol/L、10μmol/L、20μmol/L和40μmol/L;除对照组外,其它各组细胞均采用三甲基氯化锡(TMT)处理使细胞出现氧化应激状态。采用CCK-8法和荧光探针DCFH-DA法观察各组神经元的活力与细胞内活性氧(reactive oxygen species,ROS)含量的变化;TUNEL荧光染色法检测各组神经元的凋亡程度。结果:与TMT组相比,不同浓度的白藜芦醇均能提高神经元的活力,其中20μmol/L的作用最显著。20μmol/L白藜芦醇能抑制经TMT处理后神经元内的ROS水平以及凋亡程度的增加。结论:白藜芦醇具有抑制TMT诱导的大鼠海马神经元氧化损伤的作用。
AIM： To investigate the protective effects of resveratrol on oxidative damage in the rat hippocampal neurons. METHODS： The primary rat hippocampal neurons were isolated and cultured in vitro,and were treated with different concentrations of resveratrol（ 5,10,20 and 40 μmol / L） in the presence of trimethyltin chloride（ TMT）. Tetrahydrofuryl alcohol was used as a vehicle control. The cell viability was examined by CCK-8 assay. The generation of reactive oxygen species（ ROS） was determined by DCFH-DA staining. The apoptosis of the neurons was detected by TUNEL.RESULTS： Compared with TMT group,resveratrol significantly reversed the decrease in the cell viability. The viability of the neurons reached the maximum level under the condition of resveratrol treatment at 20 μmol / L. Treatment with 20μmol /L resveratrol inhibited TMT-induced increases in ROS generation and apoptosis in the neurons. CONCLUSION：Resveratrol attenuates TMT-induced oxidative damage in rat hippocampal neurons.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第6期1057-1061,共5页
Chinese Journal of Pathophysiology
基金
河南省科技厅资助项目(No.102300410136)
关键词
白藜芦醇
海马神经元
氧化应激
三甲基氯化锡
Resveratrol
Hippocampal neurons
Oxidative stress
Trimethyltin chloride
