摘要
目的:分析趋化因子CXCL17在胃癌及癌旁正常组织的表达及分布情况,并探讨其差异表达的临床意义。方法:分别采用实时荧光定量PCR和免疫组化对40例术后切除的胃癌及相应癌旁正常胃组织CXCL17的表达及分布情况进行检测。通过临床病理分析及Kaplan-Meier生存曲线的分析,明确CXCL17在胃癌组织中的表达情况及其临床意义。结果:CXCL17蛋白和mRNA的表达在胃癌组织较癌旁正常组织明显降低(P<0.05)。临床病理数据分析显示,CXCL17表达的下调与胃癌分化程度有关(P<0.01),与病人性别、年龄、淋巴结转移、原发部位肿瘤大小、肿瘤分期、癌胚抗原表达水平等未见明显相关性。Kaplan-Meier生存分析显示,CXCL17高表达组相对低表达组有明显的生存延长(P<0.05)。结论:CXCL17可作为胃癌预后的关键因素之一参与胃癌的发生发展。
AIM： To investigate the expression and distribution of chemokine CXCL17 in gastric cancer and its clinical significance. METHODS： The CXCL17 expression was detected by real-time PCR and immunohistochemistry. The correlation between the CXCL17 expression and clinicopathological features was statistically analyzed and Kaplan-Meier survival analysis was used to evaluate the prognosis. RESULTS： A lower expression levels of CXCL17 were observed in the tumor tissues compared with the paired normal tissues（ P〈0. 05）. Down-regulation of CXCL17 was associated with the degree of tumor differentiation and the size of tumor at primary site（ P〈0. 05）. Kaplan-Meier survival analysis showed that increased CXCL17 in the tumor tissues was associated with longer survival time. CONCLUSION： The result might illustrate that CXCL17 acts as a key factor in the prognosis of gastric cancer and is closely associated with the progression of gastric cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第6期1022-1026,共5页
Chinese Journal of Pathophysiology
基金
浙江省自然科学基金青年项目(No.LQ16H190003)
温州市科技计划项目(No.Y20140216
No.Y20140308)
浙江省大学生科技创新活动计划(新苗人才计划)资助项目(No.2015R413004)
