摘要
目的探讨结直肠癌中趋化因子17(CXCL17)表达与临床病理特征、预后及免疫浸润的相关性。方法选取2012年6月-2013年8月青海省人民医院肿瘤科收治的行手术治疗的68例结直肠癌患者作为研究对象,收集切除的癌组织标本及其对应的癌旁组织作为对照,采用荧光定量PCR(qRT-PCR)检测所有组织标本中CXCL17表达水平,免疫组化检测组织中CD4^+、CD8^+、CD68^+表达。结果结直肠癌组织中CXCL17表达水平和CD8^+、CD68^+阳性率高于癌旁组织,CD4^+阳性率低于癌旁组织,差异均有统计学意义(P<0.05);CXCL17表达与患者年龄、性别、TNM分期无关,差异无统计学意义(P>0.05),与分化程度、淋巴结转移相关,差异有统计学意义(P<0.05)。相关性分析显示,结直肠癌组织中CXCL17表达与CD4^+免疫细胞浸润量呈负相关性,与CD8^+、CD68^+免疫细胞浸润量呈正相关性,差异均有统计学意义(P<0.05)。CXCL17高表达患者中位生存时间、预后5年生存率均低于低表达患者,差异有统计学意义(P<0.05)。经COX多因素分析显示,淋巴结转移、CXCL17高表达是影响患者预后的独立危险因素(P<0.05)。结论结直肠癌患者癌组织中CXCL17高表达,CD4^+阳性率降低,CD8^+、CD68^+阳性率升高,CXCL17表达与患者分化程度、淋巴结转移及CD4^+、CD8^+、CD68^+免疫细胞浸润量相关,CXCL17高表达患者预后较差。
Objective To investigate the correlations between the expression of chemokine 17(CXCL17)and clinicopathological features,prognosis,and immune infiltration in colorectal cancer.Methods From June 2012 to August2013,68 patients with colorectal cancer who underwent surgical treatment in our Oncology Department were selected as the study subjects;the excised specimens of cancer tissues and their corresponding adjacent normal tissues were collected as controls.The expression of CXCL17 in all tissues was detected by fluorescence quantitative PCR(qRT-PCR);immunohistochemistry was used to detect the expressions of CD4^+,CD8^+,CD68^+.Results The expression level of CXCL17 and the positive rate of CD8^+and CD68^+in colorectal cancer tissues were higher than that in adjacent tissues(P<0.05);the positive rates of CD4^+in colorectal cancer tissues were lower than those in adjacent tissues(P<0.05);the expression of CXCL17 was not correlated with age,sex,and TNM stage(P>0.05),but with differentiation degree and lymph node metastasis(P<0.05);the correlation analysis showed that the expression of CXCL17 in colorectal cancer tissues was negatively correlated with the infiltration amount of CD4^+immune cells(P<0.05),and positively correlated with the infiltration amounts of CD8^+and CD68^+immune cells(P<0.05).The median survival time and the 5-year survival rate of patients with high expression of CXCL17 were lower than that of patients with low expression(P<0.05).COX multivariate analysis showed that lymph node metastasis and high CXCL17 expression were independent risk factors for prognosis(P<0.05).Conclusion CXCL17 was highly expressed in colorectal cancer tissues;the positive rate of CD4^+was decreased,the positive rates of CD8^+and CD68^+were increased,the expression of CXCL17 was correlated with the degree of differentiation,lymph node metastasis,and the infiltration amounts of CD4^+,CD8^+,CD68^+immune cells.The prognosis of patients with high expression of CXCL17 was poor.
作者
宋晓燕
杨娟
赞梅
SONG Xiao yan;YANG Juan;ZAN Mei(Department of Oncology,Qinghai People’s Hospital,Xining,Qinghai 810001,China)
出处
《热带医学杂志》
CAS
2019年第11期1362-1366,共5页
Journal of Tropical Medicine
关键词
结直肠癌
趋化因子17
病理特征
免疫细胞浸润
Colorectal cancer
Chemokine 17
Pathological features
Immunocyte infiltration
