摘要
Tracking of single synaptic vesicles in live neurons was considered one of challenges, but new techniques allowed tracking single synaptic vesicles in live neurons with accuracy of tens of nanometers. A synaptic vesicle loaded with a single quantum dot was tracked along with a presynaptic terminal （labeled with FM 4-64-labeled vesicles） and a postsynaptic compartment （PSD-95-GFP） in three dimensions in real time.
Tracking of single synaptic vesicles in live neurons was considered one of challenges, but new techniques allowed tracking single synaptic vesicles in live neurons with accuracy of tens of nanometers. A synaptic vesicle loaded with a single quantum dot was tracked along with a presynaptic terminal （labeled with FM 4-64-labeled vesicles） and a postsynaptic compartment （PSD-95-GFP） in three dimensions in real time.
