摘要
目的研究核素锝[^(99m)Tc]对大鼠骨髓间充质干细胞诱导分化为神经元样细胞的影响。方法采用密度梯度离心法结合贴壁培养法分离、纯化大鼠骨髓间充质干细胞,以30μmol·L^(-1)维甲酸诱导其向神经元样细胞分化,并测定核素锝[^(99m)Tc]干预骨髓间充质干细胞分化后的生物学影响。结果活度为740、1 110 MBq·mL^(-1)锝[^(99m)Tc]干预骨髓间充质干细胞6 h后,细胞存活率分别为(96.03±2.21)%、(94.47±2.12)%,锝[^(99m)Tc]干预的骨髓间充质干细胞在维甲酸诱导下可表达神经元的特异性标记物Nestin和NSE。结论常规剂量下锝[^(99m)Tc]不影响骨髓间充质干细胞的细胞活力和分化。
Objective To study the impact of [^99m Tc]to bone mesenchymal stem cells （MSCs）induced by retinoic acid to differentiate into neuron-like cell. Methods MSCs were isolated and purified by density gradient centrifugation and adherent culture,and then induced into neuron-like cell by 30 μmol·L ^-1 retinoic acid.Biologcial effects of neuron-like cell were determined after [^99m Tc]interfered in the differentiation of MSCs. Results After the interference of 740、1 1 10 MBq·mL ^-1 [^99m Tc]for 6 hours,cell survival rate was（96.03 ±2.21 ）% and （94.47 ±2.12 ）% respectively,and Nestin and NSE were expressed in the progress of 740 MBq · mL^ -1 [^99m Tc ] interfering in the differentiation of MSCs. Conclusion The cell viability and differentiation of MSCs were not affected by the conventional dose of [99m Tc]in vitro.
出处
《河南科技大学学报（医学版）》
2016年第2期84-86,共3页
Journal of Henan University of Science & Technology:Medical Science
