摘要
目的研究人骨髓间充质干细胞(mesenchymal stem cells,MSCs)向神经细胞分化的可能性。方法利用Per-coll梯度分离及贴壁筛选法分离培养和扩增MSCs;利用bFGF、化学诱导剂DMSO和BHA联合诱导MSCs向神经元转化,观察分化过程中细胞形态的变化,利用免疫细胞化学和RT-PCR方法检测神经元特异性标志物的表达情况。结果经Percoll梯度分离及贴壁筛选法获得了纯度较高的人MSCs,诱导分化后的细胞呈现双极、多极和锥形的典型神经元细胞的形态,并且分别从mRNA和蛋白水平上证明诱导分化后的细胞表达神经元标记物NSE和NF,不表达神经胶质细胞标记物GFAP。结论人MSCs可以在体外诱导分化为神经元样细胞,这种潜能使其有可能成为神经系统疾病细胞移植治疗的种子细胞。
Objective To study the potential of neural differentiation of human bone mesenchymal stem cells.Methods MSCs were isolated,cultured and proliferated by Percoll gradient centrifugation and adherence to plastic flask culture. BFGF and the chemical inductor, DMSO and BHA, were used to neural differentiation of MSCs. We observed the change in morphous and detected the identification marker expression of nerve cells by immunohistochemistry staining and RT-PCR. Results We gained high homogenous MSC, s by Pereoll gradient centrifugation and adherence to plastic flask culture.After the inducement, MSCs turned into bipolar,multipolar and taper neurons- like cells and expressed the identification marker of neurons, NSE and NF. But there was no expression of GFAP, identification marker of ghal cells. Conclusion MSCs can be induced into neuron-llke cells in vitro,this potential may represent a novel candidate resource for cellular transplant therapy of nervous system disease.
出处
《中国实验诊断学》
2007年第1期4-7,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家863重大专项(2004AA205020)
教育部博士学科点专项科研基金(20020183064)
关键词
骨髓间充质干细胞
神经元样细胞
bone marrow mesenchymal stem cells
neuron-like cells
