摘要
目的:观察口服脂联素受体激动剂(Adipo Ron)对2型糖尿病小鼠的治疗效果及对肝脏的影响。方法:40只SPF级雄性C57/BL6小鼠随机分为正常对照组和实验组,实验组给予高糖高脂饲养联合腹腔注射小剂量链脲佐菌素建立二型糖尿病(T2DM)小鼠模型,随机分为模型对照(DM)组,低剂量Adipo Ron治疗(DM+L)组,高剂量Adipo Ron治疗(DM+H)组(n=10)。检测血清中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)的变化;HE染色镜下观察肝细胞形态学变化;实时定量荧光PCR法检测肝脏中肝糖类相关基因(PEPCK)的表达。结果:与DM组小鼠比较,DM+H组和DM+L组小鼠ALT、AST、ALP、甘油三酯(TG)、葡萄糖(GLU)水平均降低(P<0.05);与DM组小鼠比较,DM+H组小鼠和DM+L组小鼠血清游离脂肪酸(FFA)浓度显著下降(P<0.05),而肝组织葡萄糖-6-磷酸酶(G-6-P)活性DM+L组小鼠显著下降,DM+H组小鼠无显著差异;与DM组小鼠比较,DM+H组小鼠肝组织磷酸烯醇式丙酮酸羧激酶(PEPCK)m RNA表达显著降低(P<0.05),而DM+L组小鼠无显著差异。结论:给予Adipo Ron治疗的小鼠血糖降低,ALT、AST、ALP的水平及G-6-P和PEPCK的表达下降,表明Adipo Ron对2型糖尿病具有显著的治疗效果,对糖尿病小鼠肝脏有一定的保护作用。
Objective： To observe the effect of Adipo Ron for the treatment of type 2 diabetes（T2DM）in mice and its effect on the liver. Methods： Forty male C57/ BL6 mice（SPF） were randomly divided to normal control（NC） group and the experimental group. To establish the T2 DM mice model,mice in the experimental group were fed with high fat and high glucose,combined with intraperitoneal injection of streptozotocin（STZ） in small doses,and mice were further subdivided into model control（DM） group,model control with low Adipo Ron（DM ＋ L） group and model control with high Adipo Ron（DM ＋ H） group（n =10）. Serum indexes,such as levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,alkaline phosphatase（ALP） were detected biochemically and the morphological changes of liver cells were observed with HE staining and expression of liver carbohydrate related gene（PEPCK） were determined by real-time fluorescence quantitative PCR（real time FQ-PCR）. Results： Compared with mice in the DM group,levels of ALT,AST,ALP,triglyceride（TG）,glucose（GLU） reduced in DM ＋ L and DM ＋ H group（P 0. 05）. Concentrations of serum free fatty acids（FFA） in DM ＋ L and DM ＋ H group reduced significantly（P 0. 05）. Besides,concentrations of liver glucose-6-phosphatase（G-6-P） in the mice of DM ＋ L group reduced significantly,while there was no significant difference in the content of G-6-P between the mice of DM ＋ H group and the mice of DM group. Furthermore,the expression of the liver phosphoenolpyruvate carboxylase（PEPCK） in the DM ＋ H group reduced significantly（P 0. 05）. Compared with the DM group no significant change was found in the PEPCK expression between DM ＋ L and DM group. Conclusion： The serum indexes such as levels of ALT,AST,ALP,TG,Glu,G-6-P and PEPCK were all reduced in DM mice treated with Adipo Ron,indicating the obvious protecting effect of Adipo Ron on the liver in DM mice.
出处
《中国应用生理学杂志》
CAS
CSCD
2016年第3期198-201,I0001,I0002,共6页
Chinese Journal of Applied Physiology
基金
2015年度浙江省公益技术应用研究计划(实验动物)项目(2015C37099)
