摘要
目的建立红参药材高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。方法 Sharpsil-T C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-水为流动相梯度洗脱;柱温为30℃;流速为1.0 ml/min;检测波长为203 nm。结果以人参皂苷Rbl为参照峰,建立10批红参的对照指纹图谱,识别出24个共有峰,其相似度均大于0.940。通过质谱推断、对照品比对确证了其中11个共有峰。结论该方法操作简便、准确可靠、重复性较好,为红参药材的质量控制提供了有效手段。
Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control. Methods Sharpsil-T C18 column（4.6 mm×250 mm,5μm）was used with acetonitrile-water in gradient elution mode. The flow rate was 1.0 ml/min, the column temperature was maintained at 30 ℃ ,and the detective wavelength was 203 nm. Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chro- matographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak. The similarity was more than 0. 940. Eleven chemical compounds were identified by comparing the reference substance. Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision, stability, and reproducibility.
出处
《药学实践杂志》
CAS
2016年第3期249-251,254,共4页
Journal of Pharmaceutical Practice
基金
上海市科学技术委员会产学研医合作项目(12DZ1970200)
关键词
红参
高效液相色谱法
指纹图谱
Ginseng Radix et Rhizoma Rubra HPLC
fingerprint
