摘要
目的探讨MAGE-A9在非小细胞肺癌(NSCLC)中的表达情况,分析MAGE-A9表达与NSCLC患者临床病理特征的关系。方法应用免疫组织化学的方法检测MAGE-A9蛋白在110例NSCLC组织及癌旁组织中的表达,通过逆转录聚合酶链反应(RT-PCR)在m RNA水平对免疫组化结果进行定量验证;对比MAGE-A9表达与NSCLC患者临床病理特征的关系,并分析MAGE-A9表达与NSCLC患者预后的关系。结果 MAGE-A9蛋白主要定位于细胞的核内,其在NSCLC组织中的高表达率明显大于癌旁组织,NSCLC组织中的MAGE-A9 m RNA水平明显高于癌旁组织,二者差异具有统计学意义(P<0.05);MAGE-A9的异常表达与患者的淋巴结状态和TNM分期有关(P<0.05);MAGE-A9低表达组患者的生存时间明显优于MAGE-A9高表达组患者(P<0.05)。结论 MAGE-A9高表达是NSCLC患者不良预后的独立危险因素,亦是NSCLC治疗的潜在靶点,值得进一步研究。
Objective The aim of this study is to investigate the expression of MAGE-A9 and significance in non-small cell lung cancer （NSCLC）. Methods The MAGE-A9 protein were detected in 110 cases primary NSCLC tissues and adjacent tissues by SP method of immunohistochemieal staining. We used quantitative real-time polymerase chain reaction （RT-PCR） analysis to further verify the results at mRNA levels. The correlation between MAGE-A9 expression, clinicopathological features and prognosis of NSCLC patients was analyzed. Results MAGE-A9 protein was mainly localized in the cell nucleus, its high expression in NSCLC tissues was significantly than the adjacent tissues, and the MAGE-A9 mRNA levels of NSCLC tissues were significantly higher than adjacent tissues, the difference was statistically significant 0a 〈0.05）.The expression level of MAGE-A9 in the primary lesions was relevant to the lymph node status and TNM stage of NSCLC patients （P 〈0.05） . The survival rate of the NSCLC patients who were positive for MAGE-A9 expression was significandy lower than that of NSCLC patients with negative MAGE-A9 expression （P 〈0.05）. Conclusion MAGE-A9 expression is an independent risk factor for poor prognosis of NSCLC patients, is also a potential target for NSCLC therapy deserves further study.
出处
《新疆医学》
2015年第12期1724-1727,共4页
Xinjiang Medical Journal
基金
新疆维吾尔自治区自然科学基金(2015211C079)
