摘要
目的探讨不同剂量郁金提取物对神经病理性疼痛模型慢性压迫性神经损伤（chronic constriction injury,CCI）大鼠痛阈及脊髓早晚期因子P-P38表达的影响及调节作用,并讨论郁金提取物对神经病理性痛的影响机制。方法选择成年健康雄性SD大鼠36只,体重250294 g,建立CCI大鼠模型,将SD大鼠随机分为：正常组,模型组,郁金提取物低、中、高剂量组,加巴喷汀组和溶媒组,每组4只,对各组大鼠进行热缩足潜伏期测定,用蛋白免疫印迹（Western blot）法检测大鼠L4L6脊髓的中早晚期因子P-P38表达。结果 1与正常组相比,造模后大鼠热缩足潜伏期（TWL）值显著下降（P〈0.05）,说明神经病理性模型造模成功;与溶媒组相比,郁金提取物高剂量组能显著上调CCI模型大鼠（Thermal withdrawal latency,TWL）值（P〈0.01）。2造模后大鼠L4L6脊髓的中早晚期因子P-P38表达灰度值与内参的比值成上升趋势,均较正常组显著增加（P〈0.01）;与溶媒组相比,郁金提取物高剂量组和加巴喷汀组能显著降低L4L6脊髓的中早晚期因子P-P38的表达（P〈0.01）。结论高剂量郁金提取物可能通过降低神经病理性疼痛CCI模型大鼠脊髓的中早晚期因子P-P38的表达而发挥抑制神经病理性疼痛的产生和发展。
Objective To investigate the effect of different doses of Radix curcumae extract onexpression of P-P38 and pain threshold of CCI rats. Methods Thirty-six healthy adult male SD rats,weight 250- 250 g,were chosen. To establish the CCI rat model,the SD rats were divided into normal group,model group,radix curcumae extract low,middle and high dose group,and gabapentin group and solvent group,and all of rats in each group were measured thermal paw withdrawal latency,using Western blot detection of rat L4-L6 spinal cord of P-P38 expression. Results 1Compared with the normal group,rats TWL value decreased significantly（ P 〈 0. 05）,indicating that neuropathic model was successfully built. Compared with the solvent group,Curcuma aromatica extract high dose group can significantly increased in CCI rats TWL value（ P 〈 0. 01）. 2After modeling,L4-L6 spinal cord in rats of P-P38 expression of gray value and the ratio of reference into an upward trend,compared with normal group increased significantly（ P 〈 0. 01）; compared with the solvent group,curcuma aromatica extract high dose group and gabapentin group can significantly reduce the L4-L6 spinal cord of P-P38 expression（ P 〈 0. 01）. Conclusion High dose of Radix curcumae extract may play a role in neuropathic pain through reducing the expression of P-P38 in spinal cord of CCI model rats.
出处
《中华全科医学》
2016年第6期934-936,共3页
Chinese Journal of General Practice
基金
浙江省中医药管理局课题(2013ZB031)
