摘要
为了探索红紫芽茶树中花青素代谢及其调控机制,应用同位素标记相对和绝对定量(iTRAQ)技术比较分析‘紫鹃’茶树同一时期不同发育程度的紫叶与绿叶的差异表达蛋白。结果表明,‘紫鹃’茶树绿叶和紫叶差异表达蛋白共鉴定出798个,其中在紫叶中上调蛋白有533个,下调蛋白有265个。这些蛋白主要参与了以下生理过程:光合作用(占14.7%)、糖代谢(5.1%)、转录调控(18.5%)、蛋白质合成(11.4%)、细胞结构蛋白(6.9%)、信号转导(6.9%)、氨基酸代谢(3.8%)、花青素代谢(3.6%)、抗氧化胁迫(3.5%)、脂代谢(3.5%)和呼吸作用(0.4%)等。‘紫鹃’茶树紫叶中可溶性总糖含量的降低可能与花青苷大量积累有关。
The mechanism regulating the anthocyanin metabolism in the reddish violet-shoot tea, Zijuan （Camellia sinensis var. kitamura）, was investigated. The isobaric tag for the relative and absolute quantification （iTRAQ） method was applied to compare the differentially expressed proteins in the purple and green leaves of the tea plant. A total of 798 differentially expressed proteins were identified from them. In the purple leaves, 533 of the proteins were up-regulated and 265 down-regulated. They were categorized by their association with photosynthesis （accounting for 14.7 ~ ）, carbohydrate metabolism （5.10% ）, transcriptional regulation （ 18.5 % ）, protein synthesis （11.4%）, structural proteins （6.9%）, signaling transduction （6.9%）, amino acid metabolism （3.8%）, anthocyanin metabolism （3.6%）, antioxidant stress （3.5%）, lipid metabolism （3.5%）, respiration, or others. It appeared that the decrease in total soluble sugars in the purple leaves of Zijuan might relate to anthocyanin accumulation.
出处
《福建农业学报》
CAS
北大核心
2016年第3期230-235,共6页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金(2014J01079)
高等学校博士学科点专项科研基金(20123515110014)
关键词
'紫鹃’茶树
ITRAQ
差异蛋白
花青素
Camellia sinensis vat. kitamura
iTRAQ
differentially expressed proteins
anthocyanin
