摘要
采用化学修饰法将酶解法获得的大米蛋白制备成硒化大米蛋白(Se-RP)并鉴定其抗氧化活性。研究表明制备的多种Se-RP均具有一定的谷胱甘肽过氧化物酶(GPX)活力,其中Se-RP24的GPX活力最高,约为116 U/g。各种Se-RP均可发挥清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟自由基(OH·)和超氧阴离子(O_2^-·)自由基的作用,并可有效维持心肌线粒体的正常功能,抑制抗坏血酸/二价铁离子(V_C/Fe^(2+))诱导损伤线粒体的膨胀,降低线粒体中脂质过氧化产物丙二醛(MDA)的含量。Se-RP的获得为大量制备植物源性硒化蛋白奠定实验基础,也为开发新型蛋白类抗氧化剂提供了新思路。
Selenide rice proteins（ Se- RP） were prepared via rice proteins obtained by enzymatic hydrolysis were chemically modified,then their antioxidant activities were identified. The results showed that Se- RPs possessed certain antioxidant activity of glutathione peroxidase（ GPX）,wherein GPX activity of Se- RP24 was highest,approximately 116 U / g. Andvarious kinds of Se- RPs could play a scavenging role in free radical such as 1,1- diphenyl-2- three nitrophenylhydrazine（ DPPH ·）,hydroxyl radical（ OH ·） and superoxide anion（ O）.Furthermore,Se- RP could effectively maintain the normal function of cardiac mitochondria,inhibit swelling of damaged mitochondria induced by ascorbic acid / divalent iron ions（ V_C/ Fe^（2＋））,and decrease content of the lipid oxidation product MDA in mitochondria.Se- RP sobtained successfully laid the experimental foundations for large scale preparation of plant- derived selenide protein,and also provided new insight for the development of new albuminoidal antioxidant drugs.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第10期165-170,共6页
Science and Technology of Food Industry
基金
吉林省教育厅"十二五"科学技术研究项目(2015-405)
吉林省科技厅科技发展计划自然科学基金项目(20150101131JC)
关键词
大米蛋白
硒
抗氧化活性
rice protein
selenium
antioxidant activity
