摘要
研究尿酸酶多囊脂质体(Uricase-multivesicular liposomes,UOMVLs)的酶学性质及其免疫原性。采用W/O/W复乳法制备UOMVLs,测定UOMVLs中尿酸酶(Uricase,UOX)的最适反应温度、最适反应p H值、热稳定性以及储存稳定性,并以其为抗原免疫SD大鼠,制备抗血清,通过间接ELISA法检测血清抗体效价。UOMVLs和UOX最适反应温度均为40℃;最适反应p H值分别为8.0和8.5;UOMVLs的稳定性明显高于UOX;UOMVLs和UOX的血清抗体效价分别为1∶500和1∶8 000。因此UOMVLs的酶学性质明显优于游离UOX,免疫原性明显低于游离UOX,为该酶的临床应用提供了实验依据。
This study aimed to investigate the enzymatic property and immunogenicity of uricase-multivesicular liposomes （UOMVLs）. UOMVLs were prepared by a double emulsion method, and optimal temperatures, pH values, storage and thermal stabilities of UOMVLs and uricase （UOX） were detenlained. SD rats were immunized using UOMVLs as antigens, and their serum antibody titers were determined. Results showed that the optimal temperatures for UOMVLs and U0X were both 40 ℃, while the optimal pH values were 8.0 and 8.5, respectively. The stability of UOMVLs was significantly higher than that of UOX. The serum antibody titers of rats immunized with UOMVLs and UOX were 1：500 and 1：8 000, respectively. Therefore, the enzymatic property of UOMVLs was significantly better and the immunogenicity was obviously lower than UOX, which provided an experimental basis for the clinical application of UOX.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2016年第4期364-369,共6页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(30973645)
重庆市首批高等学校优秀人才资助计划
关键词
尿酸酶
多囊脂质体
酶学性质
免疫原性
uricase, multivesicular liposome, enzymatic property, immunogenicity
