摘要
2014年11月以来,我国部分地区所饲养的肉鸭发生了以雏鸭发育迟缓,上下喙萎缩,舌头外伸为特征的疾病。根据病变,暂将该病称为鸭短喙-侏儒综合征。为确定引起该病的病原,本研究从山东聊城发病鸭群采集14份病料样品,其中包括舌及各脏器组织样品。经PCR方法检测,所有发病鸭肝脏、脾脏中均可检出细小病毒,将其中5份阳性病料样品处理后接种9日龄鸭胚,分离得到1株鸭源细小病毒分离株(命名为SD株)。此外,该分离株的659 bp的Rep和VP1核苷酸序列与鹅细小病毒和番鸭细小病毒进行遗传进化分析,结果表明SD株与鹅细小病毒具有相近的遗传进化关系。将该分离株人工感染1日龄樱桃谷肉鸭,可以复制出相似病例。结果表明,从鸭短喙-侏儒综合征自然病例分离到的一株鸭源鹅细小病毒,该病毒可能与鸭短喙-侏儒综合征有关。
Since November in 2014, an infectious disease has emerged in many duck farms in some regions of China, which characterized by serious growth retardation and short bills with protruding tongues. Based on the pathological changes, the disease was tentatively designated beak atrophy and dwarfism syndrome （BADS）. In this study, fourteen samples including tongue and organ tissues were collected from the diseased ducks in Liaocheng, Shandong province, and all liver and spleen samples were duck parvovirus positive detected by PCR. In addition, a virus strain was isolated from spleen tissue sample with 9-day-old duck embryos, and PCR results showed that the isolate was positive for duck parvovirus, designated SD. Sequence analysis based on a 659 bp fragment of the virus DNA, partial of Rep and VP1 gene sequence of SD strain revealed that the isolate was most closely related to goose parvovirus. Moreover, the clinical signs of BADS were able to be reproduced in one-day-old Cherry Valley Peking ducks by artificial infection with the isolate of a duck parvovirus. These evidences indicated that the BADS might be caused by duck parvovirus infection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2016年第5期348-351,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
河北省研究生创新资助项目
河北农业大学大学生创新创业训练计划项目
