摘要
目的:考察细辛凝胶剂中甲基丁香酚与马兜铃酸A的经皮吸收特性。方法:以SD大鼠腹部离体皮肤为渗透屏障,应用Franz扩散池进行离体经皮吸收实验,高效液相色谱法测定甲基丁香酚与马兜铃酸A的含量。结果:本实验方法下得出甲基丁香酚的时间-累积透过量方程为:Q=0.029 4t-0.008 9,相关系数r为0.990 7,稳态渗透速率J为0.029 4 mg/cm^2/h。细辛凝胶剂接受液中未检测出马兜铃酸A。结论:初步阐述甲基丁香酚的经皮吸收特性与马兜铃酸A的经皮含量,为细辛凝胶剂多组分经皮吸收规律研究奠定基础。
Objective： To make a study of the characteristic of percutaneous absorption of methyl eugenol and aristolochic acid A from asarum gels. Methods： We used skin in vitro of SD rats as permeability barrier and carried on a permeation experiment with Franz permeability cell. We determined the contents of methyl eugenol and aristolochic acid A by HPLC method. Results： Under this experimental method,the equation of the methyl eugenol time- the amount of accumulation was as follows： Q = 0. 029 4t- 0. 008 9; correlation coefficient r = 0. 990 7; steady- state penetration rate J = 0. 0294 mg/cm^2/h. We did not detect the aristolochic acid A from accept liquid of asarum gels. Conclusion： This experiment obtained the characteristic of percutaneous obsorption methyl eugenol and percutaneous content of aristolochic acid A,meanwhile providing the base for subsequent asarum gels multicomponent percutaneous absorption research.
出处
《中华中医药学刊》
CAS
北大核心
2016年第5期1179-1182,共4页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅科学技术研究项目(L2013375)
关键词
细辛凝胶剂
甲基丁香酚
马兜铃酸A
经皮吸收
asarum gels
methyl eugenol
aristolochic acid A
percutaneous absorption
