摘要
目的建立骨痨敌薄膜衣片中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和黄芪甲苷的含量测定方法。方法采用高效液相色谱（HPLC）法,色谱柱为Hypersil GOLD C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水,梯度洗脱,流速1.0 m L/min,检测波长203 nm,柱温25℃,进样量5μL。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和黄芪甲苷分别在0.108~1.62μg（r=0.999 7）、0.409~6.135μg（r=0.999 8）、0.108 8~1.632μg（r=0.999 9）、0.503~7.545μg（r=0.999 7）范围内线性关系良好;平均回收率分别为99.74%、98.87%、99.31%、98.66%,RSD分别为1.31%、1.47%、0.96%、1.45%。结论本方法简便可靠,重复性好,可用于骨痨敌薄膜衣片中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和黄芪甲苷的质量分数测定。
Objective To establish a quantitative determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and astragaloside Ⅳ in Gulaodi filmlok. Methods HPLC was performed on the Hypersil GOLD C18column（ 250 mm × 4. 6 mm,5 μm） and the mobile phase was acetonitrile-water with gradient elution at a flow rate of 1.0 m L / min. The detection wavelength was 203 nm. The column temperature was 25℃ and the injection volume was 5 μL. Results The linear ranges of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and astragaloside Ⅳ were 0. 108-1. 62 μg（ r = 0.999 7）,0. 409-6. 135 μg（ r = 0.999 8）,0.108 8-1.632 μg（ r = 0. 999 9）,0. 503-7. 545 μg（ r = 0. 999 7）,respectively. The average recovery of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and astragaloside Ⅳ were 99. 74%,98. 87%,99.31%,98.66% with RSD of 1.31%,1.47%,0.96% and 1.45%,respectively. Conclusion The method is simple,reliable and reproducible. It could be used for the quantitative determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and astragaloside Ⅳ in Gulaodi filmlok.
出处
《广东药学院学报》
CAS
2016年第2期192-195,共4页
Academic Journal of Guangdong College of Pharmacy
基金
陕西省咸阳市科学技术研究发展计划项目(2012K06-07)
