摘要
目的:研究复叶耳蕨总黄酮(total flavonoids from Arachniodes exilis,TFA)对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的作用及其分子机制。方法:通过茜素红染色、碱性磷酸酶(alkaline phosphatase,ALP)活性考查BMSCs成骨情况;RT-PCR检测Ⅰ型胶原酶α1(collagen typeⅠα1,COL1A1)、骨钙蛋白(osteocalcin,OCN)、骨桥蛋白(osteopotin,OPN)、BMP2、BMP4、Runx2、Osterix转录水平;ELISA检测骨形态发生蛋白(bone morphogenetic protein,BMP)信号通路相关蛋白BMP2、BMP4、Runx2表达情况。结果:TFA能显著增加BMSCs碱钙结节的形成、ALP活性,上调COL1A1、OCN、OPN的表达,促进BMSCs成骨分化,而Noggin能抑制该作用;TFA能显著上调BMP2、BMP4、Runx2、Osterix的表达。结论:TFA可能通过BMP信号通路促进大鼠BMSCs成骨分化。
Objective： To investigate the effects and its molecular mechanism of the total flavonoids from Arachniodes exilis（ TFA）on the osteogenic differentiation of bone marrow mesenchymal stem cells（ BMSCs）. Methods： Alizarin red staining and alkaline phosphatase（ ALP） activity were used to evaluate the osteogenic differentiation capacity of BMSCs; RT-PCR was used to assess the transcription level of collagen type Ⅰ α1（ COL1A1）,osteocalcin（ OCN） and osteopotin（ OPN）,BMP2,BMP4,Runx2,Osterix; the expression of bone morphogenetic protein（ BMP） signaling related proteins（ BMP2,BMP4,Runx2） were detected by ELISA. Results： TFA enhanced osteogenic differentiation capacity of BMSCs significantly,demonstrated by increasing the formation of calcium nodules,ALP activity and gene expression of COL1A1,OCN and OPN,while those effects were dramatically attenuated by treatment with Noggin; TFA significantly upregulated the expression of BMP2,BMP4,Runx2 and Osterix. Conclusion： TFA promoted the osteogenic differentiation capacity of rat bone marrow mesenchymal stem cells through BMP signaling.
出处
《中药材》
CAS
CSCD
北大核心
2016年第1期150-154,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金计划项目(81160536)
江西省教育厅科技课题资助项目(20142BBG70018)
关键词
复叶耳蕨总黄酮
骨髓间充质干细胞
成骨分化
BMP信号通路
Total flavonoids from Arachniodes exilis
Bone marrow mesenchymal stem cells
Osteogenic differentiation
BMP signaling
