摘要
目的探讨柴油机排放颗粒(DEP)对急性冠状动脉综合征(ACS)患者与非冠状动脉粥样硬化性心脏病(NCHD)患者外周血单个核细胞(PBMC)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)表达的影响。方法采用密度梯度离心法分离ACS和NCHD患者PBMC,用不同浓度的DEP(0、10、50、100 mg·L-1)刺激PBMC 24 h。收集PBMC培养液上清,用酶联免疫吸附试验法检测PBMC培养液上清中IL-1β和TNF-α水平。结果随着DEP浓度的增加,PBMC释放IL-1β和TNF-α的量也随之增高,其中以50 mg·L-1组水平最高(P<0.05);在同一DEP浓度下,ACS患者PBMC中IL-1β和TNF-α的相对增加量较NCHD患者高,提示ACS对DEP毒性作用易感。结论 DEP刺激可增加受试患者尤其是ACS患者的PBMC产生过量炎性因子。
Objective To investigate the effect of diesel exhaust particles（ DEP） on the expression of including interleukin-1β（ IL-1β） and tumor necrosis factor-α（ TNF-α） in peripheral blood monocytic cells（ PBMC） of acute coronary syndrome（ ACS） and non-coronary heart disease（ NCHD） patients. Methods The PBMC from ACS and NCHD patients were purified through density gradient centrifugation. The PBMC were incubated with different concentrations of DEP（ 0,10,50,100 mg·L- 1） for 24 h. The supernatants were collected and the IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. Results With increasing of DEP concentration,the IL-1β and TNF-α expressions in PBMCs were increased,which peaked at the dose of 50 mg·L- 1（ P〈0. 05）. At the same concentration,DEP could induce more production of IL-1βand TNF-α in the PBMCs from ACS patients than that from the NCHD patients,it implied that ACS patients were more susceptible to DEP stimulation. Conclusion DEP stimulation increases the over expression of IL-1β and TNF-α in the PBMCs from the ACS and NCHD patients,especially the former.
出处
《新乡医学院学报》
CAS
2016年第4期288-290,296,共4页
Journal of Xinxiang Medical University
基金
国家自然科学基金面上项目(编号:81573112
81373030)
河南省教育厅科技创新团队项目(编号:14IRTSTHN017)
新乡医学院研究生科研创新支持计划(编号:YJSCX20446Y)
