摘要
背景:斑块内血管新生是在动脉粥样硬化病变基础上发生的,而血管新生又促进粥样硬化病变的发展;可溶性血管内皮生长因子受体1基因转染减少兔血管损伤后的新生内膜形成,亦显著减少早期血管的炎症和增生以及晚期的新生内膜形成。目的:观察局部高表达的可溶性血管内皮生长因子受体1对动脉粥样硬化斑块的作用。方法:雄性新西兰大白兔48只,随机分为4组。1正常对照组(n=8)。2假手术组(n=8)。3球囊损伤+高脂饮食+p EGFP-N1组(n=16)。4球囊损伤+高脂饮食+p EGFP-N1-s Flt-1组(n=16)。前2组兔给予正常颗粒饲料饮食。后2两组给予高脂饮食,2周后行髂动脉球囊损伤血管内皮,球囊损伤+高脂饮食+p EGFP-N1组和球囊损伤+高脂饮食+p EGFP-N1-s Flt-1组兔右髂动脉腔分别注射空质粒p EGFP-N1和脂质体包裹的可溶性血管内皮生长因子受体1重组质粒。结果与结论:球囊损伤+高脂饮食+p EGFP-N1组以及球囊损伤+高脂饮食+p EGFP-N1-s Flt-1组兔血脂水平明显升高,造模3 d后髂动脉可溶性血管内皮生长因子受体1表达水平明显增加,均出现不同程度的动脉粥样硬化斑块形成,且球囊损伤+高脂饮食+p EGFP-N1-s Flt-1组兔斑块面积、斑块周径、斑块最大厚度和斑块内阳性细胞数量明显少于球囊损伤+高脂饮食+p EGFP-N1组。表明可溶性血管内皮生长因子受体1基因在动脉粥样硬化斑块动物模型中可有效表达并抑制动脉粥样硬化斑块的发生、延缓斑块的发展。
BACKGROUND： Angiogenesis in atherosclerotic plaque occurred on the basis of atherosclerotic lesions, and the new formed blood vessels promoted the development of angiogenesis. Soluble vascular endothelial growth factor receptor 1 （sFlt-1） gene transfection reduces neointimal formation after vascular injury in rabbits, also reduces early vascular inflammation and proliferation, and the formation of neointima lately. OBJECTIVE： To observe the effects of highly expressed sFlt-1 on atherosclerotic plaque. METHODS： A total of 48 male New Zealand rabbits were divided into four groups randomly： normal control group （n=8）, sham operation group （n=8）, bal oon injury＋high lipid diet＋pEGFP-N1 group （n=16） and bal oon injury＋high lipid diet＋pEGFP-N1-sFlt-1 group （n=16）. Normal control group and sham operation group were supplied with normal diet. Bal oon injury＋high lipid diet＋pEGFP-N1 group and bal oon injury＋high lipid diet＋pEGFP-N1-sFlt-1 group were supplied with high lipid diet and injured by bal oon 2 weeks later. Bal oon injury＋high lipid diet＋pEGFP-N1 group was transfected with pEGFP-N1 and bal oon injury＋high lipid diet＋pEGFP-N1-sFlt-1 group was transfected with pEGFP-N1-sFlt-1. RESULTS AND CONCLUSION： In the bal oon injury＋high lipid diet＋pEGFP-N1 group and bal oon injury＋high lipid diet＋pEGFP-N1-sFlt-1 group, blood lipid levels apparently increased. At 3 days after model establishment, sFlt-1 expression levels noticeably increased, and atherosclerotic plaque formed to different degrees. Plaque area, plaque perimeter, plaque maximum thickness and the number of positive-cel s within the plaque were significantly less in the bal oon injury＋high lipid diet＋pEGFP-N1-sFlt-1 group than in the bal oon injury＋high lipid diet＋pEGFP-N1 group. These results confirm that sFlt-1 gene may express effectively in iliac artery wal of rabbit, inhibited the formation of atherosclerotic plaque and delayed the development of atherosclerotic plaque.
出处
《中国组织工程研究》
CAS
北大核心
2016年第18期2697-2702,共6页
Chinese Journal of Tissue Engineering Research
