摘要
目的探讨艾滋病病毒(HIV)抗体蛋白印迹试验(WB)结果为不确定的标本,其HIV-1病毒载量检测的情况,为WB结果不确定标本的诊断提供参考依据。方法选取WB结果为不确定的血浆标本,且4周后随访到受检者,采集第二份血浆标本进行WB检测。对首份标本进行HIV-1病毒载量检测。综合对比分析两种方法的检测结果。结果共59例WB结果为不确定的标本。21例后期随访WB结果为阳性的标本中,病毒载量检测结果大于检测限的标本19例,低于检测限(TND)的标本2例。4例随访WB难以确证的标本,通过病毒载量检测做出诊断。34例后期随访WB结果为阴性的标本,其病毒载量结果全部为TND。病毒载量检测对此类标本的敏感性为96.61%(57/59),特异性为100%。结论病毒载量检测对HIV早期感染的诊断具有重要意义,尤其是对孕产妇和WB随访难以判断的标本。对于WB结果出现两条或两条以上核心条带的标本,随访WB阳转和病毒载量浓度较高。但病毒载量TND并不能完全排除艾滋病感染的可能。
Objective To investigate accuracy of viral load（VL）test in detection of uncertain HIV antibody,to provide evidence for diagnosis of uncertain HIV-1antibody.Method The samples with uncertain HIV antibody by Western-blot（WB）test were collected,and followed up to get their blood samples again four weeks later for the retest by WB.The first collected samples with unclear WB results got viral load detection for the confirmed diagnosis by comparing the results of the two methods.Results A total 59 cases of blood samples showed unclear WB test results,with 21 cases proved to be HIV positive by follow-up WB test,VL test results of 19 samples above the detection limit,and 2cases of detection results as TND.4cases with uncertain results even by follow-up WB test got confirmed diagnosis by VL test.34 cases got confirmed diagnosis of negative HIV antibody by follow-up WB test,and their VL were all TND.The sensitivity and specificity of the VL test were 96.61%（57/59）,and 100%respectively.Conclusion VL testing is important for diagnosis of HIV infection,especially for pregnant women and those difficult to diagnose by follow-up WB test.Two or more than two core bands in the tests show more positive results of WB and VL test.However,the TND result of VL cannot necessarily be extrapolated that a person is not infected with HIV.
出处
《中国艾滋病性病》
CAS
北大核心
2016年第4期237-240,共4页
Chinese Journal of Aids & STD
