摘要
目的:探索高血压患者发生颅脑外伤后脑组织中血管紧张素Ⅱ1型受体(AT1R)表达水平及其与脑水肿、预后的相关性。方法:选取颅脑外伤合并高血压患者45例为观察组,另选非高血压的颅脑外伤手术患者45例为对照组,两组均于术中收集因挫伤被清除的脑组织,分别采用RT-PCR和Western blot法测定脑组织中AT1R mRNA及蛋白水平,于术后第1、3、5天行头颅CT检查,通过CT值反映脑水肿程度,术后3月采用格拉斯哥预后分级(GOS)评价高血压患者和非高血压患者的预后差异性。结果:两组在AT1R mRNA水平、蛋白水平和脑水肿程度差异有统计学意义(P<0.05),观察组更低;两组在预后评分上差异无统计学意义(P>0.05)。结论:原发性高血压患者在颅脑外伤后脑组织中AT1R的m RNA和蛋白表达、脑水肿程度明显高于非高血压的颅脑外伤患者,预后评分无明显差异。
Objective： To explore the patients with hypertension cerebral vascular brain injury in angiotensin II type 1 receptor（AT_1R） expression and its correlation with cerebral edema and prognosis.Methods： A total of craniocerebral trauma complicated with hypertension patients with 45 cases as the observation group,select non hypertension patients with cerebral trauma 45 cases as control group,two groups were in operation collection due to contusion were cleared from the brain tissue,RT-PCR and Western blot were used in brain tissues were determined by AT_1R mRNA and protein levels and in the postoperative 1,3 and 5 days after CT and by CT value to reflect the degree of brain edema and postoperative 3 months by Glasgow Outcome Score（GOS） evaluation of patients with hypertension and non hypertension patients differences in prognosis.Results： The two groups in AT_1R m RNA and protein level and cerebral edema degree were statistically significant（P〈0.05）,the observation group were lower in group two; prognosis score had no significant difference（P〈0.05）.Conclusion： The expression of m RNA and protein in patients with essential hypertension AT_1R in brain tissue of brain injury and brain edema in patients with traumatic brain injury was significantly higher than that of non hypertension,there were no significant differences in the prognosis.
出处
《中国医药导刊》
2016年第4期367-368,370,共3页
Chinese Journal of Medicinal Guide
