摘要
目的探索血管紧张素Ⅱ1型受体(AT1R)基因多态性与高血压脑出血后AT1R表达水平及其与脑水肿的相关性。方法收集重庆医科大学附属永川医院神经外科2011年12月至2012年8月住院的高血压脑出血手术患者45例为观察组,对照组选取难治性癫痫手术患者45例,每组患者均采集静脉血后检测AT1R的基因多态性,术中取被出血毁损挫伤的脑组织,分别采用RT-PCR和Western blot法测定脑组织中AT1RmRNA及蛋白水平,于术后第1、3、5天行头颅CT检查,通过CT值反映脑水肿程度。结果观察组AC/CC基因型与AA基因型AT1RmRNA水平表达差异无统计学意义(P>0.05),AC基因型术区CT值比AA基因型更低,差异有统计学意义(P<0.05);对照组各基因型AT1RmRNA、蛋白水平和脑水肿程度差异无统计学意义(P>0.05)。结论 AT1R基因多态性与脑组织中AT1RmRNA的表达无明显相关性,AT1R蛋白水平和脑水肿程度有相关性。
Objective To investigate the correlation of angiotensin Ⅱ type 1 receptor （AT1R） gene polymorphism with AT1R expression levels and brain edema after hypertensive intracerebral hemorrhage .Methods 45 operative patients with hypertensive intracerebral hemorrhage in the Affiliated Yongchuan Hospital of Chongqing Medical Univercity from December 2011 to August 2012 were collected as the experimental group and 45 operative patients with refractory epilepsy weres selected as the control group .The venous blood in the two groups were collected for detecting the AT 1R gene polymorphism ;The brain tissue was taken from lesions in operation ,then AT1R mRNA concentration was determined by RT-PCR and the AT1R protein level was determined by Western blot ;Head CT was performed on postoperative 1 ,3 ,5 d;the degree of cerebral edema was reflected by CT value . Results The levels of two kinds of genotype AT1R mRNA in the experimental group had no statistically significant difference（P〉0 .05）;the operative area CT value of AC genotype was significantly lower than that of AA genotype with statistical difference （P〈0 .05）;the ATIRmRNA of various genotypes ,protein level and cerebral edema in the control group had no statistical differences . Conclusion The AT 1R gene polymorphism has no obvious correlation with the concentration expression of AT 1R mRNA in the brain tis-sue;there is correlation between AT 1R protein level and AT 1R protein level and the cerebral edema degree in the brain tissue .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第21期2691-2694,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30973087)
重庆市卫生局科学计划项目资助(2011-2-290)
关键词
颅内出血
高血压性
受体
血管紧张素
1型
脑水肿
基因多态性
intracranial hemorrhage, hypertensive
receptor, angiotensin, type 1
brain edema
gene polymorphism
