摘要
目的:评价补骨脂异黄酮对人成纤维细胞体外创伤模型的影响。方法:不同浓度(4.5、9、18、36μg/m L)补骨脂异黄酮处理人成纤维细胞。分别用MTT法、羟脯氨酸法、实时定量PCR法测定补骨脂异黄酮对细胞活力、胶原合成、环氧化-2和诱导型一氧化氮合成酶表达的影响。通过补骨脂异黄酮对过氧化氢诱导的细胞损伤保护作用和自由基清除能力的测定,确定补骨脂异黄酮抗细胞氧化的效果。结果:本研究的结果表明,补骨脂异黄酮预处理成纤维细胞具有显著保护由过氧化氢引起的细胞损伤(P<0.05)。36μg/m L补骨脂异黄酮处理细胞12 h后细胞总抗氧化能力显著增加(P<0.05)。此外,补骨脂异黄酮预处理人类成纤维细胞可显著抑制脂多糖(LPS)诱发的COX-2 mRNA的表达(P<0.01)。成纤维治疗组和对照组之间细胞增殖和胶原合成没有明显的差异(P>0.05)。结论:补骨脂异黄酮通过其抗氧化和抗炎作用可能是一种有效的皮肤创伤治疗剂。
Objective： To evaluate the effect of corylin on human fibroblast cells in an in vitro model of wound healing.Methods： Human fibroblast cells were treated by different concentrations（ 4. 5,9,18,36 μg / m L） of corylin. The effects of corylin on cell viability,collagen synthesis,expression of cyclooxygenase-2（ COX-2） and inducible nitric oxide synthetase（ i NOS） were assessed by 3-（ 4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazoliumbromide,hydroxyproline analysis and real-time PCR,respectively. The effect of corylin on cellular antioxidant status was determined by protection against hydrogen peroxide（ H2O2）-induced cell injury and free radical scavenging activity of cells. Results： It showed the protected cells were against H2O2-induced injury（ P〈0. 05）. After the 12 h treatment of cells it indicated that with the pretreatment of fibroblast cells withcorylin significantly of 36 μg / m L corylin,the total antioxidant capacity of cells significantly increased（ P〈0. 05）. Furthermore,pretreatment of human fibroblast cells with corylin significantly inhibited lipopolysaccharide（ LPS）-induced COX-2 mRNA expression（ P〈0. 01）. There was no significant difference in fibroblast proliferation and collagen synthesis between treatment and control groups（ P〉0. 05）. Conclusion： Corylin may be useful as a therapeutic agent for the treatment of cutaneous wounds through its effects of antioxidation and anti-inflammation.
出处
《中医药学报》
CAS
2016年第2期37-40,共4页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金面上项目(81274035)
黑龙江省自然科学基金面上项目(D201234)
黑龙江省博士后科研启动金(LBH-Q13162)
黑龙江中医药大学校基金(2012-035082)
黑龙江中医药大学佳木斯学院院级项目(201501)
关键词
抗氧化
胶原合成
环氧酶-2
Antioxidant capacity
Collagen synthesis
Cyclooxygenase-2
