摘要
目的探讨不同级别宫颈上皮内瘤变(CIN)患者宫颈上皮内p16、p53及Ki-67表达的差异。方法采用免疫组化方法检查CIN患者(其中CIN I级73例、CIN II级79例、CIN III级96例)和正常宫颈上皮(40例)内p16、p53及Ki-67的表达。结果 p16在正常宫颈上皮组织、CIN I、II、III级患者宫颈组织中表达的阳性率分别为17.5%、64.4%、94.9%和97.9%;而p53在各组相应的阳性率分别为20.0%、20.5%、40.5%和38.5%;Ki-67在各组相应的阳性率分别为30.5%、58.9%、94.9%和96.9%。与正常宫颈上皮组织相比,p16和Ki-67在CIN I级组织中表达的阳性率明显升高(P<0.05或0.01);p16、p53和Ki-67在CIN II级和III级组织中表达的阳性率亦高于正常宫颈组织(P<0.05或0.01);与CIN I级相比,CIN II级和III级的p16、p53及Ki-67表达阳性率明显升高(P<0.05或0.01)。结论 p16、p53及Ki-67水平的检测有助于CIN的早期诊断和分级。
Objective To investigate the difference in expression of p16, p53 and Ki-67 in cervical epithelium in patients with cervical intraepithelial neoplasia（CIN） of different grades, and to evaluate their clinical significance. MethodsImmunohistochemica method was used to detect the expression of p16, p53 and Ki-67 in cervical epithelium of patients with CIN（96 patients with CIN III, 79 patients with CIN II and 73 patients with CIN I） and in normal cervical epithelium（40 patients）.Results The positive expression rate of P16 in patients with normal cervical epithlium, and patients with CIN I, CIN II and CIN III was 17.5%, 64.4%, 94.9% and 97.9%, respectively; the positive expression rate of P53 in each group was 20%, 20.5%, 40.5% and 38.5%, respectively; and the positive expression rate of Ki-67 in each group was 30.5%, 58.9%, 94.9% and 96.9%, respectively. Compared with normal cervical epithelium, the positive expression rate of p16 and Ki-67 in patients with CIN I was significantly higher（P〈0.05 or 0.01）, while the positive expression rate of p16, p53 and Ki-67 in patients with CIN II and III was significantly higher（P〈0.05 or 0.01）; and compared with the patients with CIN I, the positive expression rate of p16, p53 and Ki-67 in patients with CIN II and CIN III were significantly higher（P〈0.05 or 0.01）. Conclusion The detection of p16, p53 and Ki-67 level is helpful to the early diagnosis and grading of CIN.
出处
《广东医学院学报》
2015年第5期565-568,共4页
Journal of Guangdong Medical College
