摘要
目的 :通过对人原NOB1及泛素C(ubiquitin C,UBC)在人肺癌细胞及肺癌组织中表达情况的研究,探讨NOB1与UBC分子与肺癌发生的关系,并探讨这两个分子之间的相互关系。方法 :采用实时逆转录酶-聚合酶链式反应(quantitative real-time polymerase chain reaction,q RT-PCR)检测NOB1 m RNA的表达情况;利用免疫组织化学法检测NOB1表达情况;使用免疫印迹技术(Western Blot)检测NOB1及UBC蛋白的表达情况;并在肺癌细胞株中过表达和干扰UBC后,检测NOB1蛋白的表达情况。结果:各组肺癌细胞中NOB1 m RNA及UBC蛋白的表达水平均高于正常肺组织细胞BEAS-2B(F=9.874,P<0.05);NOB1及UBC在肺癌组织中的表达明显高于癌旁组织(F=11.234,P<0.05)和正常组织(F=9.116,P<0.05);过表达UBC使NOB1表达增加,干扰NOB1使NOB1表达降低。结论 :NOB1及UBC1与肺癌发生相关,UBC可能促进NOB1表达,引起肺癌增殖。
Objective：This study was designed to examine the expression of NOB1 and UBC in human lung cancer cells and tissues with a focus on their relations to the occurrence of lung cancer and the relationship between these two molecules was also discussed. Methods：The m RNA expression of NOB1 gene was detected by using quantitative real-time polymerase chain reaction（q RT-PCR） method. Immunohistochemical staining was carried out to measure expression of NOB1 in tissue. Protein levels of NOB1 and UBC were determined in cells and tissues and aftert ransfection of UBC plasmid by Western Blot.Results： Compared with normal human lung cancer cell BEAS-2B, NOB1 m RNA expression and UBC protein expression were increased significantly（F=9.874,P〈0.05）. NOB1 and UBC expressions in lung cancer tissues were distinctly higher than adjacent non-neoplastic tissues and normal tissues（F=11.234,9.116,P〈0.05）. UBC overexpression increased the expression of NOB1, while NOB1 expression can be almost abolished after UBC-silence. While si UBC significantly downregulated NOB1.Conclusions： NOB1 and UBC have positive correlations with the occurrence of lung cancer. UBC may upregulate NOB1 to promote the proliferation of lung cancer.
出处
《南通大学学报（医学版）》
2015年第6期525-529,共5页
Journal of Nantong University(Medical sciences)
基金
中国博士后基金资助项目(2013M541705)
江苏省博士后基金资助项目(1301072C)
江苏省六大人才高峰课题(YY-006)
关键词
非小细胞肺癌
NOB1
泛素C
增殖
non-small cell lung carcinoma
NOB1
ubiguitin C
proliferation
