摘要
目的 观察细胞外信号调节激酶5（ERK5）在结肠癌及癌旁正常组织中的表达并分析其与临床相关病理参数间的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶（SP）技术及反转录-聚合酶链反应（RT-PCR）法检测106例结肠癌组织及距离病灶10 cm处的正常结肠黏膜组织中ERK5及其mRNA的表达,并且分析ERK5的表达与临床相关病理参数间的关系.结果 106例结肠癌组织中ERK5的阳性表达率及其mRNA的表达水平（75.47％;1.44±0.01）均高于癌旁正常组织（11.32％;0.54 ±0.02）.结肠癌组织中ERK5的表达水平与病灶侵袭深度、分化程度、临床分期及是否有淋巴结转移有关.ERK5阳性表达患者的24个月生存率（22.20％）与阴性表达患者（43.67％）比较降低.结论 ERK5的阳性表达与结肠癌的发生有关.
Objective To investigate the expression and significance of extracellular signal-regulated kinase5 （ERK5） in colon cancer and adjacent normal mucosa.Methods Positive expression of ERK5 protein in colon cancer and adjacent normal mucosa were detected by immunohistochemical streptavidin-peroxidase （SP）.The expression level of ERK5 mRNA was analyzed by reverse transcriptase-polymerase chain reaction （RT-PCR）.Relationships between ERK5 protein expression and clinicopathological characteristics were analyzed.Results Positive expression rate of ERK5 protein and its mRNA expression level in 106 cases colon cancer （75.47%;1.44 ± 0.01） were higher than that in adjacent normal mucosa （11.32%;0.54 ± 0.02）.The ERK5 protein positive expression rates was statistically significant in patients with different elements of differentiated degrees,depth of invasion,Tumor Node Metastasis （TNM） staging and lymphatic metastasis.The 24 months survival rate in patients with ERK5 protein positive expression （22.20%） was lower than those in patients with ERK5 protein negative expression （43.67%）.Conclusion ERK5 protein positive expression is in relation to the occurrence of colon cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第4期1106-1108,共3页
Chinese Journal of Experimental Surgery
关键词
结肠癌
细胞外信号调节激酶5
免疫组织化学
Colon cancer
Extracellular signal regulated kinase -5
Immunohistochemistry
